7 Questions 2 Answers 0 Followers
Questions related from Austin Morrissey
How do you effectively choose a cell line for murine xenografts? What information is needed to effectively compare several cell lines? Is there a way to predict what cell lines will produce...
18 October 2022 3,601 3 View
Most immunofluorescence protocols I've seen apply DAPI as the last step in staining, following the application of primary and secondary antibodies. If DAPI was added first, could it interfere...
14 October 2022 8,110 2 View
Can proximal and distal tubules be distinguished with relative certainty in immunofluorescence?
07 October 2022 9,283 1 View
A common negative control setup in immunofluorescence is the omission of primary antibodies. What purpose does a negative control serve in these contexts? Is it just to get an idea of the...
20 April 2022 4,057 3 View
I'd like to measure levels of NF-κB activation. Is nuclear localization of NF-κB sufficient evidence of activation in human cells?
18 April 2022 9,768 1 View
Hello I am looking for a suitable adipocyte cell line to treat with human sera. It seems that human adipocyte cell lines are rarer than those of mice (3T3-L1). Is there an advantage to a human...
18 February 2022 3,370 3 View
How does autofluorescence factor into antibody selection in immunofluorescent microscopy? How do you distinguish it visually?
13 January 2022 1,918 3 View
