Hello al
Please i want to manually prepare antibiotic disk for disk diffusion method for an antibacterial plus enzyme inhibitor ( as amoxiclave for example)
My question is that how disk can be prepared? First mix the fractions then put on the disk or individually?
Thanks in advance
I prepared them one day to test bacterial crude extracts! if I remember you have to buy celullose discs (or cut them from a sheet of cellulose paper (with a piece cutter)) you put the disc on the plate then you put a volume of your ATB solotion (not more than 100µL it will overflow) and it's done!
I hope it's helpful
Kindly the following RG links, they are very useful:
https://www.researchgate.net/post/How-to-prepare-our-own-disk-for-disk-diffusion-study
https://www.researchgate.net/post/How_to_prepare_antibiotics_disk
The well diffusion assay is suitable for aqueous samples because they are difficult to dry on paper discs
. A suspension of each isolate was made at turbidity equal to 0.5 McFarland standards and then plated on Muller- Hinton agar. Antibiotic disc
Do you want to say that you want the best method to measure the synergistic action between an enzyme and an antibiotic on the tested organism?
Mahmoud A. Abushiba Hello dear Dr.Mahmoud, yes I want to test the synergistic effect between antibacterial combinations and antibacterial alone on tested organism
This is our recently published paper that may help you. If you have difficulty understanding the experiment, I can help you. There are other experiments that can be conducted regarding synergy. I am in the process of making summaries in this regard.
I wish you all the best.
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