Response Surface modeling with insignificant p values?

I ran the response surface modeling using central composite design. And I got this output. Can I take this model? The model is having lack of fit p value more than 0.05. But other parameters are...

01 February 2020 1,364 3 View

Why mice not rats are used for testing efficacy of vaccine?

What is the reason that most of the researcher uses mice for evaluating immune response of vaccines. Why nobody uses rat for the same purpose?

10 November 2019 4,837 2 View

Can I use EDTA instead of EGTA for P22 Phage transduction?

Hi Colleagues, I am going for transduction of Salmonella Typhimurium. As per the protocol available with me the method goes in the following way: 1. Take 100 ul of overnight grown culture of...

08 September 2019 8,164 2 View

No PCR amplification in large volume of PCR mix?

I am doing PCR amplification of 8.5 kb DNA fragment. I have observed a wierd result. When I use a master mix of 50 micL, I get the amplification but when I use 100 micL reaction it does not give...

01 February 2019 7,380 5 View

How to differentiate statistically different groups in different days?

In my experiment, I have four groups namely A, B, and C. I have made the observations in all three groups in 4 different days namely 7th , 14th, 21st and 28th day. Now, I want to perform a...

04 May 2018 8,445 11 View

How to denote the letters in mean on the basis of p value in R-console?

I have performed ANOVA (1 way) followed by Turkeys Multiple comparison in R console. Now I need to denote letters to the means in table to show if there is any significant difference between the...

08 September 2017 234 4 View

How to remove the acetone from suspension of GantrezAN Nanoparticle

I am preparing Gantrez Nanoparticle dissolved in Acetone. At final stage I am to remove the Acetone. I remove the Acetone by vaccume rotary evaporator at 40OC at 555 mmHg vaccume pressure....

07 August 2017 231 3 View

How to calculate the cutoff value of Indirect ELISA?

I am doing indirect Elisa with test serum sample and negative serum samples. Someone please let me know how to calculate the cut-off value to determine the titre.

07 August 2017 4,550 4 View

How can I perform repeated measure anova with post hoc multiple comparison in R?

I need to do repeated measure anova with post hoc multiple comparison in R. I am attaching a hypothetical data "Mice.csv". I have four groups namely IA, IB, IIA, and IIB. In all the groups one...

07 August 2017 7,978 3 View

How to perform Tukey's pair-wise multiple comparison test after repeat measure ANOVA in R?

I am doing the Repeat Measure ANOVA using a below mentioned model in R. But I am not understanding how to perform pair-wise multiple comparison. I have added one hypothetical data in the file...

01 February 2017 793 6 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Can anyone advise me about a cortisol antibody made in rabbit to set up an ELISA?

I am trying to set up a cortisol EIA using mouse anti-rabbit IGg as coating and a rabbit cortisol antibody as secondary ab. I am searching for a sensitive and specific cortisol Ab made in rabbit

20 February 2021 3,366 5 View