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Questions related from Shantanu Tamuly
I ran the response surface modeling using central composite design. And I got this output. Can I take this model? The model is having lack of fit p value more than 0.05. But other parameters are...
02 February 2020 1,473 3 View
What is the reason that most of the researcher uses mice for evaluating immune response of vaccines. Why nobody uses rat for the same purpose?
11 November 2019 4,930 2 View
Hi Colleagues, I am going for transduction of Salmonella Typhimurium. As per the protocol available with me the method goes in the following way: 1. Take 100 ul of overnight grown culture of...
09 September 2019 8,257 2 View
I am doing PCR amplification of 8.5 kb DNA fragment. I have observed a wierd result. When I use a master mix of 50 micL, I get the amplification but when I use 100 micL reaction it does not give...
02 February 2019 7,518 5 View
I am doing indirect ELISA for estimation of specific IgM antibodies in poultry chicken. I would like to know what should be dilution of anti-chicken IgM HRP conjugate that should I take for...
06 June 2018 5,056 3 View
In my experiment, I have four groups namely A, B, and C. I have made the observations in all three groups in 4 different days namely 7th , 14th, 21st and 28th day. Now, I want to perform a...
05 May 2018 8,538 11 View
I have performed ANOVA (1 way) followed by Turkeys Multiple comparison in R console. Now I need to denote letters to the means in table to show if there is any significant difference between the...
09 September 2017 317 4 View
I am preparing Gantrez Nanoparticle dissolved in Acetone. At final stage I am to remove the Acetone. I remove the Acetone by vaccume rotary evaporator at 40OC at 555 mmHg vaccume pressure....
08 August 2017 318 3 View
I am doing indirect Elisa with test serum sample and negative serum samples. Someone please let me know how to calculate the cut-off value to determine the titre.
08 August 2017 4,721 4 View
I need to do repeated measure anova with post hoc multiple comparison in R. I am attaching a hypothetical data "Mice.csv". I have four groups namely IA, IB, IIA, and IIB. In all the groups one...
08 August 2017 8,091 3 View
Whenever I do more than 3 factor ANOVA in R, it says "ANOVA F-tests on an essentially perfect fit are unreliable". I use the command > anova(lm(y~T*conc*cat*gam)) Where the gam, T, cat and conc...
02 February 2017 735 5 View
I am doing the Repeat Measure ANOVA using a below mentioned model in R. But I am not understanding how to perform pair-wise multiple comparison. I have added one hypothetical data in the file...
02 February 2017 908 6 View
I am trying to prepare the SDS PAGE gel. But the problem is the separating gel is highly sticky and does not look like a regular gel. Then apart from this the stacking gel is not getting...
04 April 2015 8,717 10 View
I am trying to isolate outer-membrane vesicles from Salmonella Typhimurium using ultracentrifuge method. My protocol is as follows: 1. The Salmonella Typhimurium is grown upto end of...
01 January 1970 1,454 9 View
