A good in vitro model for iron deficiency?

07 August 2018 453 0 View

No title

07 August 2018 5,265 1 View

DNase digestion of mitochondrial DNA when extracting mtRNA from whole blood?

Hi all, I'm soon going to extract RNA from whole blood (PAXgene Blood RNA extraction kit, Qiagen), and we're considering checking mitochondrial gene expression in qPCR. From what I read in the...

05 June 2018 4,140 1 View

A silly question regarding buffer exchange columns?

Hi, I have a silly question regarding buffer exchange columns (in that case the zebda desalting columns, from thermo scientific). So as I understand the process is pretty straight forward : 1....

08 September 2017 9,107 1 View

Cloning human bikunin (aka UTI) kunitz domain

Hi, I'm currently trying to clone Kunitz domain from the human Bikunin (represented below). As I'm not too much use to cloning I was wondering very basic questions : 1) Shall I start my primers at...

06 July 2017 9,934 7 View

Can somebody recommend a good paper for Cancer models in mice?

Hi all, I'm going to start soon with a cancer model in mice, using subcutaneous injection of PancO2, LLC, B16-F10 and 4T1 cells. I'm trying to get the maximum information out of each experiment by...

31 December 2016 9,834 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View