Hi all,
I'm soon going to extract RNA from whole blood (PAXgene Blood RNA extraction kit, Qiagen), and we're considering checking mitochondrial gene expression in qPCR. From what I read in the literature, mtDNA contamination might be an issue, and so I was wondering if special measure should be taken when digesting DNA during RNA extraction (extended incubation time, using a special type of enzyme...)?.
Thank you in advance for any information regarding this subject!
Hi Henry
as DNase will only digest double stranded nucleic acids and since mtDNA is double stranded, no need to have special treatment for whole DNA degradation. you just need to be sure that the cell membrane treatment will treat at same time the mitochondrial membrane, but in general there is no issue on that point.
fred
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