Henri Weidmann

7 Questions 14 Answers 0 Followers

Questions related from Henri Weidmann

A good in vitro model for iron deficiency?

Hi all, I´m currently screening the literature for in vitro model of iron deficiency. From what I read so far, it seems that letting cells in a serum free media for 72h or using an iron chelator...

08 August 2018 515 0 View

Does 3´RNAseq requires the same RNA integrity than traditional RNAseq?

Hi all, I guess everything is in the title: would you expect (or better yet was it shown) that 3´RNAseq, where only the last 50-75 BP of the transcript are sequenced, to be affected by RNA...

08 August 2018 5,325 1 View

DNase digestion of mitochondrial DNA when extracting mtRNA from whole blood?

Hi all, I'm soon going to extract RNA from whole blood (PAXgene Blood RNA extraction kit, Qiagen), and we're considering checking mitochondrial gene expression in qPCR. From what I read in the...

06 June 2018 4,210 1 View

A silly question regarding buffer exchange columns?

Hi, I have a silly question regarding buffer exchange columns (in that case the zebda desalting columns, from thermo scientific). So as I understand the process is pretty straight forward : 1....

09 September 2017 9,171 1 View

Cloning human bikunin (aka UTI) kunitz domain

Hi, I'm currently trying to clone Kunitz domain from the human Bikunin (represented below). As I'm not too much use to cloning I was wondering very basic questions : 1) Shall I start my primers at...

07 July 2017 10,021 7 View

Difference between B16 and B16V?

Hi all, Does anyone knows the difference between the B16 and B16V cell lines? Tanks!

01 January 2017 9,037 3 View

Can somebody recommend a good paper for Cancer models in mice?

Hi all, I'm going to start soon with a cancer model in mice, using subcutaneous injection of PancO2, LLC, B16-F10 and 4T1 cells. I'm trying to get the maximum information out of each experiment by...

01 January 2017 9,903 4 View