we tried to use this SPr to check the binding between spike protein and aptamer. we used SA, CM, NTA chips. still, we couldn't produce any data. do anyone here use this machine to check aptamer and protein binding?
How to synthesis manganese doped graphene oxide after the synthe of graphene oxide by Hummers method ?
04 June 2023 3,844 3 View
Growing population demand can only be filled by AI or new technologies, rather any sustainable approaches should dominate to save the nature.
31 May 2023 3,739 3 View
I'm trying to make a precusor that's liquid at room temperature. TOPO dissolves it at high temp but liquify at RT. Is there a solvent that can dissolve stearic acid that’ll stay liquid at RT.
12 January 2023 9,694 1 View
This is in context of the objective function of a multivariate optimization problem say, f(a,b,c). I am looking for a "measure" for the degree of bias of f(a,b,c) towards any of the input variables.
15 November 2022 7,522 4 View
I want to use size exclusion chromatography for drug-encapsulated PLGA nanoparticles. I am going to use Sephadex. but I couldn't find any suitable procedure for nanoparticle separation using SEC....
14 November 2022 1,379 2 View
I synthesized above polymer and tried to incorporate FAM labeled aptamer to that without any chemical conjugation. then I checked fluorescent count in supernatant and the precipitate . And also I...
04 November 2022 1,427 0 View
I Try to process V3 but I can't find a proper way to do it.
16 January 2022 4,450 3 View
In my lab, I'm using the BI4500 biosensing SPR instrument. But still, I couldn't generate any results. Does anyone here uses BI45000 SPR?
10 September 2021 5,517 0 View
i am trying to find KD of aptamer as the analyte and protein. I'm using a CM sensor chip. I'm using PBS with tween 0.005% and 1 mM MgCl2 as RB. Normally I don't use ethanolamine to block the...
10 July 2021 7,917 5 View
Celebrity endorsements are common across lifestyle products even basic things as groceries are being endorsed by celebrities, but when it comes to investing does endorsement of a mutual fund...
17 May 2021 6,127 4 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I am looking for software to help analyze SELEX NGS data, including alignment, sequence enrichment, and other related tasks. Can anyone recommend suitable tools or software? Best wishes, Waleed
30 July 2024 1,061 5 View
Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...
30 July 2024 6,794 0 View
I work with planar interdigitated electrodes (IDEs). The finger width and the spacing between the fingers are 5 micron. The surface of the IDEs is coated by a non-conductive polymer layer...
16 July 2024 5,031 1 View
Hi, I am doing aptamer based electrochemical biosensor. First, I cleaned screen printed gold electrode using 0.5M H2SO4. Then, immobilized aptamer followed by blocking using 2mM MCH. I use...
06 July 2024 9,852 1 View
Hello. I have try to immobilize the thoilated aptamer onto the gold surface such as Gold coated silicon wafer and the gold coated screen printed electrode. However, it is failed, after aptamer...
29 April 2024 4,054 1 View
What is the best way to obtain high-performance aptamers after performing sequence amplification by asymmetric PCR other than biotin and streptavidin?
17 April 2024 6,973 2 View
I used the autodock4 tool for docking the aptamer and the ligand. I prepared the DNA aptamer following the process: sequence -> predict the 2d structure by MFold -> predict the 3D structure...
31 March 2024 2,772 0 View
molecular docking, aptamer
27 March 2024 4,622 2 View
I have confusion in required Temperature.
25 March 2024 3,200 1 View