In my lab, I'm using the BI4500 biosensing SPR instrument. But still, I couldn't generate any results. Does anyone here uses BI45000 SPR?
How to synthesis manganese doped graphene oxide after the synthe of graphene oxide by Hummers method ?
04 June 2023 3,844 3 View
Growing population demand can only be filled by AI or new technologies, rather any sustainable approaches should dominate to save the nature.
31 May 2023 3,739 3 View
I'm trying to make a precusor that's liquid at room temperature. TOPO dissolves it at high temp but liquify at RT. Is there a solvent that can dissolve stearic acid that’ll stay liquid at RT.
12 January 2023 9,694 1 View
This is in context of the objective function of a multivariate optimization problem say, f(a,b,c). I am looking for a "measure" for the degree of bias of f(a,b,c) towards any of the input variables.
15 November 2022 7,522 4 View
I want to use size exclusion chromatography for drug-encapsulated PLGA nanoparticles. I am going to use Sephadex. but I couldn't find any suitable procedure for nanoparticle separation using SEC....
14 November 2022 1,379 2 View
I synthesized above polymer and tried to incorporate FAM labeled aptamer to that without any chemical conjugation. then I checked fluorescent count in supernatant and the precipitate . And also I...
04 November 2022 1,427 0 View
I Try to process V3 but I can't find a proper way to do it.
16 January 2022 4,450 3 View
we tried to use this SPr to check the binding between spike protein and aptamer. we used SA, CM, NTA chips. still, we couldn't produce any data. do anyone here use this machine to check aptamer...
16 December 2021 8,842 0 View
i am trying to find KD of aptamer as the analyte and protein. I'm using a CM sensor chip. I'm using PBS with tween 0.005% and 1 mM MgCl2 as RB. Normally I don't use ethanolamine to block the...
10 July 2021 7,917 5 View
Celebrity endorsements are common across lifestyle products even basic things as groceries are being endorsed by celebrities, but when it comes to investing does endorsement of a mutual fund...
17 May 2021 6,127 4 View
I work with planar interdigitated electrodes (IDEs). The finger width and the spacing between the fingers are 5 micron. The surface of the IDEs is coated by a non-conductive polymer layer...
16 July 2024 5,031 1 View
I'm trying to produce silver nanoparticles using plant extract, but I didn't observe the expected peak in the UV-Vis spectrum between 380-420 nm, where silver nanoparticles typically appear. After...
04 May 2024 6,463 11 View
Hello together, I am looking for a conversion factor for BLI (Bio-layer interferometry) from the nm values into a more comparable pg/mm2? For SPR it seems to be common to convert the RU values...
13 March 2024 6,238 0 View
Respected scholar, I have synthesized bare silver nanoparticles using sodium borohydride reduction at room temperature and measured their UV-Vis absorption spectrum. The spectrum showed two...
26 January 2024 3,191 3 View
I need help with selection ADA confirmatory assay format. Does anyone have the experience of selection confirmatory assay among ELISA, SPR?
14 January 2024 8,923 0 View
SPR
11 December 2023 3,727 3 View
I tested two analytes with the same a.a sequence, just different by 1a.a to one target protein. My SPR outcome looks like the attached figure below. Can I say the right one binds stronger than...
09 November 2023 6,681 2 View
Cytiva sells a GST antibody as part of a rather expensive kit for coupling tagged proteins to their sensor chips. The antibody can not be purchased separately, and we do not need all the other...
26 October 2023 3,800 1 View
When I fuse the multi-single-multi fiber structure, coat it with silver film to stimulate the SPR phenomenon, and establish the transmission spectrum, I add water to the sensing area. The spectral...
23 October 2023 6,782 1 View
If we place a SPR sensor device between the electromagnet and varying the intensity of electric and magnetic field. How does sensor behaves especially when external electric field and evanescent...
23 October 2023 3,941 2 View