Siddhartha Thennakoon

6 Questions 2 Answers 0 Followers

Questions related from Siddhartha Thennakoon

Solvent that dissolve stearic acid?

I'm trying to make a precusor that's liquid at room temperature. TOPO dissolves it at high temp but liquify at RT. Is there a solvent that can dissolve stearic acid that’ll stay liquid at RT.

13 January 2023 9,687 1 View

Can size exclusion chromatography used for nanoparticles?

I want to use size exclusion chromatography for drug-encapsulated PLGA nanoparticles. I am going to use Sephadex. but I couldn't find any suitable procedure for nanoparticle separation using SEC....

15 November 2022 1,349 2 View

Aptamer encapsulation to PLGA-PEG-PLGA?

I synthesized above polymer and tried to incorporate FAM labeled aptamer to that without any chemical conjugation. then I checked fluorescent count in supernatant and the precipitate . And also I...

05 November 2022 1,403 0 View

Did anyone use BI-4500 5-Channel SPR System from Biosensing Instrument to check the binding between aptamer and protein?

we tried to use this SPr to check the binding between spike protein and aptamer. we used SA, CM, NTA chips. still, we couldn't produce any data. do anyone here use this machine to check aptamer...

17 December 2021 8,833 0 View

May I ask- SPR Problem?

In my lab, I'm using the BI4500 biosensing SPR instrument. But still, I couldn't generate any results. Does anyone here uses BI45000 SPR?

11 September 2021 5,504 0 View

Can aptamer bind to activated reference channel in SPR like a normal SPR binding?

i am trying to find KD of aptamer as the analyte and protein. I'm using a CM sensor chip. I'm using PBS with tween 0.005% and 1 mM MgCl2 as RB. Normally I don't use ethanolamine to block the...

11 July 2021 7,908 5 View