What tool should i use for aptamer - small ligand docking?

More Huy Do's questions See All

Dear fellow researchers studying in molecular biology, are there any tips on how to avoid plasmid dimerization when extracting plasmids?

Using INTRON's DNA-spin Plasmid DNA purification kit to extract plasmids from specific E.Coli strains, my team and I have encountered numerous plasmid dimerizations that lead to over-weighted...

23 July 2024 3,490 6 View

The reason for breeding Loxp mice with FLPe mice?

I am planning to use CD4 Cre mice crossed with Loxp mice. However, the laboratory from which I am trying to get the loxp sperm says they have "the product of the EMMA ko crossed with the FLPe to...

16 July 2024 7,475 1 View

Zero pivot in Abaqus concrete 3D printing simulation using model change?

Recently I've been working on a simulation of concrete 3D printing, and has successfully simulated a layer-by-layer 3D printing process using model change. However, when I proceeded to try...

06 July 2024 2,130 0 View

How to solve Bubble Error in fiber splicing?

I'm having a bubbling error while splicing 100/350 um optical fiber (core/cladding) on the Fujikura FSM100P+. I have tried some ways such as changing Prefuse power and Prefuse time but to no...

03 July 2024 7,463 6 View

How to use the BLAST primer to check the possible bonds of primer?

Hi everyone, I run PCR to checked presence of specific gene in Clostridium perfringens but I received 2 bands, one band showed product size expected and another one showed higher product size. I...

31 May 2024 645 1 View

What statistical test to use to compare the response of two groups after a treatment?

In my study i have 2 groups of patient (diagnosed with stage 1 and 2 at the beginning of treatment), where dependent variable is "lesion volume", which was measured before and after treatment....

27 May 2024 10,115 11 View

DAB fail-regions in DAB positive area. Why?

I am recently observing DAB staining fail-regions (red circles or red arrows on pictures) on my DAB stained slides. Does anyone have ideas why I am getting this? I can see only hematoxylin stained...

16 May 2024 693 0 View

Does mixed values of a variable (positive and negative value) affect the result of SPSS analysis?

Im testing the change in lesion volume between disease stages. I have measured lesion at T0 and T1, and calculated the change in volume with formula: (VolumeT1 - VolumeT0) / VolumeT0 * 100 In my...

15 May 2024 4,382 8 View

How to find fully funded PhD scholarship in the subject of spatial and/or regional economics?

Hello researchers and professors I'm from Pakistan and have published few articles in health economics and poverty from spatial perspectives. I have good expertise in spatial analysis and now I...

30 April 2024 3,048 2 View

[LC-HRMS] Mass higher than normal for Intrinsicaly disordered domain?

For my project, I am attempting to purify an intrinsically disordered domain cloned in a pET28b vector. The strain used for overexpression is Rosetta II(DE3). Firstly, I encountered an unexpected...

23 April 2024 9,939 4 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Seeking Software Recommendations for SELEX NGS Data Analysis?

I am looking for software to help analyze SELEX NGS data, including alignment, sequence enrichment, and other related tasks. Can anyone recommend suitable tools or software? Best wishes, Waleed

30 July 2024 1,061 5 View

How to prediction 2D to 3D aptamer?

Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...

30 July 2024 6,794 0 View

How to restart MD without using checkpint file in GROMACS?

Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar

29 July 2024 3,084 8 View

AutoGrid and Autodock 4.2.6 window opens for 1 second then closes and there is no output files?

Good day, I am a student trying to work on Autodock for a project regarding Ligand-DNA interaction so i am quite new to molecular docking. i have followed tutorials and did all the steps...

28 July 2024 2,136 4 View