Why ICP-MS test for Teflon filters that collected PM2.5, show really high results in the millions of ug/g ?

I analyzed soil and ash samples using ICP-MS for 28 metals, then I resuspended those samples to collect PM2.5 particles in a filter using a selective inlet inlet. My only issue that Fe and Al...

13 February 2021 7,592 3 View

Hello every one .. I want to know the wave length of sotalol and sertraline determination by UV spectrophotometer ?

i want the wave length that detected bu uv spectro not by other devices

18 January 2021 7,020 2 View

Can I solve 2d heat equation by Euler's forward and backward method! How?

I need to know what are the steps of solving the 2d heat equation, and can I implement it in Python (especially with Euler's method).

01 April 2020 7,028 4 View

What are the manufactures of polymer containing carbon nano tubes ?

What is the best industrial method for manufacturing polymers containing carbon nano-tubes composites materials . if there is any existing companies dealing with such composites , please let me...

19 January 2019 963 3 View

How to get the longitudinal-optical (LO) phonon energy?

in midm-infrared quantum cascade lasers

11 October 2016 160 3 View

Is there any techniques to measure thermal stability of polymer instead of TGA ?

Help please

20 November 2014 299 5 View

What is the difference between quenching my PET or cooling it slowly?

19 November 2014 1,277 4 View

Why CNTs nuclear polymer stronger than GNP ?

09 November 2014 3,393 7 View

What does tan delta curve height mean in the polymer Nanocomposite?

I am testing polymer composites via DMA. Could please any one help?

29 May 2014 9,373 8 View

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IS IT ABLE TO CUT CHAIN INFECTION WITH COVID19? BY INHIBITING ADHENCE TO EPITHELIAL CELLS?

01 January 1970 6,180 28 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Why tick's salivary gland extract didn't run in the gel (SDS PAGE )??

Greetings, I want to make SDS PAGE for my sample (Tick salivery gland extract), I tried concentrations 10%, 12%. I also tried high molecular weight ladder. But the protein stopped at the top and...

25 February 2021 7,698 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View