Did anyone treated the sample with trypsin in SDS-PAGE?

More Fahdah Alshammari's questions See All

Hi please how can i find quasi- experimental sample size calculator to compute the exact number ?

i need sample size calculator for quasi study design

02 June 2024 9,822 2 View

Do you suggest that I order my kit from BT Lab?

I need to order an ATP Assay Kit for my work, and I found this kit on the BT Lab website. Has anyone tried the kits from this site? Because this is the first time I will order from them.

27 February 2024 6,940 1 View

How can estimation nonlinear phase shift φNL in optical fiber?

Hello I asked about nonlinear phase shift φNL in optical fiber. How can estimation φNL? and how the power effected on it?

29 September 2022 8,850 2 View

Why ICP-MS test for Teflon filters that collected PM2.5, show really high results in the millions of ug/g ?

I analyzed soil and ash samples using ICP-MS for 28 metals, then I resuspended those samples to collect PM2.5 particles in a filter using a selective inlet inlet. My only issue that Fe and Al...

13 February 2021 7,973 3 View

Hello every one .. I want to know the wave length of sotalol and sertraline determination by UV spectrophotometer ?

i want the wave length that detected bu uv spectro not by other devices

18 January 2021 7,272 2 View

Good evening ... I want a scientific paper about the cultivation of microalgae from soild waste?

soild waste such as plants leaves , food waste , plant flowers

05 October 2020 3,615 3 View

Can I solve 2d heat equation by Euler's forward and backward method! How?

I need to know what are the steps of solving the 2d heat equation, and can I implement it in Python (especially with Euler's method).

01 April 2020 7,144 4 View

What are the manufactures of polymer containing carbon nano tubes ?

What is the best industrial method for manufacturing polymers containing carbon nano-tubes composites materials . if there is any existing companies dealing with such composites , please let me...

19 January 2019 1,189 3 View

How to get the longitudinal-optical (LO) phonon energy?

in midm-infrared quantum cascade lasers

11 October 2016 288 3 View

Is there any techniques to measure thermal stability of polymer instead of TGA ?

Help please

20 November 2014 445 5 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

What is the cause of this aggregation just bellow 70 kDa mark?

Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...

21 July 2024 5,128 4 View

Less substrate signals towards the last lanes of the Native PAGE?

I have been running native page for FAM DNA substrate ( fluorescence samples) for protein DNA binding reaction. Binding is there but towards the end of the lane , I am loosing signals...

17 July 2024 6,213 4 View

Anybody can assist me in Protein and magnetic beads seperation?

Hi, I hope you are doing well, I am working to remove DNA from protein sample by using silica hydroxode magnetic beads. I used different binding buffers but I din't get my protein band in initial...

02 July 2024 5,502 4 View

How accurate is Image J for SDS bands analysis?

I want to check my protein expression but the sample (target protein) on my SDS PAGE shows a little difference as compared to the control. I measured the band density through IMAGE J but the band...

01 July 2024 1,841 3 View

How to troubleshoot error E1 on Biorad Powerpac Basic despite using sufficient and fresh buffer?

I have used this BIorad Powerpac Basic for 4 years now, before which it was used for another 5-6 years. Generally we addressed the E1 error by compensating with fresh buffer and/or filling the...

01 July 2024 7,104 1 View

Thin DNA gel loading sample tips?

I have a 0.25mM thick gel and I am having trouble cleaning out the wells to ensure constant sample application. I usually use a thin syringe to get into the wells and rinse them out with running...

01 July 2024 2,124 2 View