How to design ion NIPT in Ion Proton NGS?

1. Does thermofisher provide ready to use primer mix for NIPT? or the user has to define the list of primers? 2. Did ion community already design NIPT? 3. What is the method of analysis? 4. what...

09 October 2017 840 1 View

What was the first cancer for which genetic marker was identified?

06 July 2017 6,993 4 View

What will happen, if any alkaline earth metal is used in a PCR reaction mix instead of MgCl2?

Magnesium is an alkaline earth metal and its electron configuration is 2,8,2. All other alkaline earth metal such as calcium(2,8,8,2), strontium(2,8,18,2), barium(2,8,18,18,2) has a similar...

07 August 2016 2,129 5 View

What are the low impact factor cancer journal?

07 August 2016 9,400 0 View

What are the excitation spectra of N-Methyl mesoporphyrin in a flurometer?

In my current study, I am using NMM as fluorescence material. Most of the previous study mentioned the excitation spectra of NMM is 399nm and the emission spectra is 610nm. Is it possible that I...

06 July 2015 5,903 4 View

Molecular Genetic Pathology. ISBN: 978-1-58829-974-1 (print) 978-1-59745-405-6 (online)

Hi everyone, I need a little help out here. I need the above mention book. If any one have the soft copy of this book and if you may upload the book over research gate, it would be very...

07 August 2014 1,146 3 View

How to accurately identify cancer tissue origin with MicroRNAs?

In this article fig. 1. The Author has made a decision tree with 24 nodes. Each node is specified for a specific cancer tissue of origin and the couple of MicroRNA which can identify these cancer...

05 June 2014 5,962 4 View

Is it necessary to have the same type of samples in a case control study?

For an example: I want to study rsxxxxxxx SNP in Indian population. I have FFPE samples obtained from NSCLC patients. Which kind of control samples should I procure? Is it necessarily to be a FFPE...

04 May 2014 5,089 16 View

How do I calculate desired sample size for a case control study?

For an example, I want to study rs2910164 in Indian NSCLC patients. How do I calculate the desired sample size to get a significant power in the study?

04 May 2014 4,815 8 View

How can I differentiate or identify metastatic cancer cell from a non metastatic cancer cell?

Is there any gold standard method of identifying a metastatic cancer cell from a non metastatic cancer cell? Is there any molecular assay available to differentiate these two type of cancerous cell?

04 May 2014 6,071 6 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View