In this article fig. 1. The Author has made a decision tree with 24 nodes. Each node is specified for a specific cancer tissue of origin and the couple of MicroRNA which can identify these cancer tissues of origin. My question is, if I isolate miRNAs at the node14(hsa-miR-21, let-7e), node21(hsa-miR-205, 152), node24 (hsa-miR182, 34a, 148), node10(hsa-miR-194, 382, 210), will it be enough to identify cancerous tissue originated from the lung.
Why am I asking this question, because, I want to identify cancerous tissue, which has migrated to different region but originated in the lung. So if I take miRNAs from those specified nodes, will it be enough to identify lung cancer tissue, which has migrated to different region but originated in the lung.
Article MicroRNAs accurately identify cancer tissue origin
Been busy being (trying) to be a "patient" getting tired of the way things are done out here (Chicago). You wouldn't believe half of it. Maybe you would. I guess we're all human, but it's amazing how some Drs. That used to impress me with their knowledge and intelligence disgust me by their actions. Im sick, I'm dying soon, all I csre about is my son and wanting to make sure he knows how precious life is, but all he continuously sees is my pain and insanity of trying to get and pay for help I would joyously give a stranger if I could
Hi
I dont think any part of the mirs collection will do, nor do I think that this paper is a valid diagnostic assay.
Rosetta genomics took this work and create a valid diagnostic assay out of it.
you can search for latter papers of mine, I am not with the company and more and I think you should contact them
good luck
Shai
Hi
i think the specific receptor or noncoding (Specificity) of tumour site using RT-PCR
Dear Sangeet Bhaumik
microRNAs have to provide potential signature model for various cancers and other diseases. While miRNA in terms of sequencing is difficult , though being done provides huge number of miRNA in a specific cancer as you have exemplified some in your discussion. Few important learning points:
1- Specific sets of miRNAs usually express or otherwise together and they are termed miRNA families.
2-Some miRNA have the potential to act as pan cancer biomarkers but still no specificity is provided.
3-Some biomarkers are like positive or negative acute phase reactant and may rise or fall with non-cancerous disease.
4-There is some but as i experienced little correlation between blood and tissue based miRNAs
5- The science of miRNAs is still emerging and a lot more has to be learnt i guess before they are available, if available for clinical use.
6-Also need to have complete data about pre, pri and miRNA and he cleaving proteins like DROSHA, DICER and factors incorporated in RISC complex.
The whole picture is yet to appear and but hope is there that someday they may be appearing as both for diagnostic use and therapeutic targets like Riversin (spelling ?) for treating hepatitis C.
So potential is there but more research is needed to quantify and deal associated aspects of miRNA
Sorry, that I could not help you straight as i interpret the knowledge about this subject is still evolving.
Kind regards
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