I wanted to do that but I havn't found any single literature/forum evidence of that. I would really appreciate your input!
Hi there, I have never heard of someone doing that - but the question is, why would you like to do that? If you are afraid that your sorted cells are not yielding good RNA, then the problem is most likely not an RNAse sitting in the flow cell...
The typical cleaning cycle (Bleach/EtOH) would certainly remove trace RNAses that would be present. Can't imagine why you would need the certainty of RNAZap.
@Florian Mair: You are correct, I wanted to do that as my sorted nuclei (no cells) are not yielding good RNA. Do you have any insights on what could be the reason?
@Craig Silver: I also thought the same. FACSclean should be enough. But as I am not getting desired RNA amount, that's why, I wanted to do it.
Well as said, FACSclean (i.e. bleach) should take care of any RNAses sitting around in the flow cell. If you are having trouble getting RNA, I would rather have a critical look at all the reagents and handling steps before and after, and potentially at the sheath fluid and buffers used. I would suspect that the culprit is sitting somewhere there... Also, you can make sure that you clean the collection block and any areas surrounding the sorter with RNAZap. Hope you can figure it out!
@Florian Mair: Thanks a lot! I am thinking of cleaning the Sheath tank properly and prepare the machine for aseptic sort as per the guide book. Also, I have to adjust the protocol a bit as I am assuming, my nuclei were probably not surviving due to inappropriate storage buffer. Anyways, highly appreciate your input!
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