I am measuring nuclear total RNA from mouse embryonic cortical tissues. I have done the bioanalyzer and for all the samples, it shows the rRNA ratio from 3.7 to 5.0, which is way beyond the theoretically possible value(2.6). My gut feeling tells, the RNA is not degraded (as 28s rRNA peak is intact, and there are no smears on the lower sized rna), but why would it be the case? Any suggestion would be highly appreciated!
Thanks again in advance!
- I wouldnt worry too much you cannot see any degradation
- It could be something specific and peculiar to your embryonic tissue source
- Irrespective I would proceed as a normal
You can run your RNA on the gel to see how it looks. If it is degraded, you will see it easily.
- I wouldnt worry too much you cannot see any degradation
- It could be something specific and peculiar to your embryonic tissue source
- Irrespective I would proceed as a normal
@ Leonid Ouchanov : Thank you for your comment. But my RNA amount is too low to see on a gel run. It is about 10ng in total.
@ Laurence Stuart Dawkins-Hall : Thank you for your comments. I also think likewise. I am planning to proceed with the sequencing.
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