A DNA microarray (also commonly known as DNA chip or biochip) is a collection of microscopic DNA spots attached to a solid surface. Scientists use DNA microarrays to measure the expression levels of large numbers of genes simultaneously or to genotype multiple regions of a genome.
micro array as a high-throughput method is very helpful in determination of miRNA profile of different disease.
What species are you working on? We've used affymetriex miRNA microarray (genechip.4.0 ) (http://www.affymetrix.com/catalog/131473/AFFY/miRNA-Array#1_1) This array has probes for every miRNA recorded in miRBase (more than 30k mature miRNAs + some small RNAs). Make sure you have the proper scanner to process these arrays.
Thanks Shuai
I'm working on human serum and PBMC samples and search for human and viral miRNA. By the way how much was the price of kit?
Around 300 US dollars per chip only. You also need another kit to process the array. We sent our samples to a Affymetrix contract lab and it cost us 500 bucks per chip including everything but not analysis. You can contact Affymetrix to see if there is any lab in your region.
Hi,
I have done my work at Exiqon. There facility is more reliable. I will suggest to go with Exiqon. They have custom made chips for your selected miRNAs.
Thanks Ram
Does it work with any scaners? What type of scaner did you worked with?
Regargs
Hello Davod,
Custom-made chips are the best option for you, I feel.
Warm regards,
Venk
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