Can I use several qualitative traditions at the same time for my Master thesis?

Hello, I am doing a research paper in which I will have to do semi-structured interviews to a group of SMEs from my hometown to analyze and define their needs and interests. Which tradition of...

27 January 2021 6,140 3 View

Only my PCR primers are not working?

I am working on an undergraduate project within a group. The topic is to identify carbapenamase resistnace gene in K.pneumoniae and E.coli. The target genes are OXA-48, KPC, IMP and VIM. We are...

26 November 2020 6,992 5 View

Good book providing a survey of international agricultural development?

Hello all, What recommendations do you have of books providing a strong survey of key issues impacting international agricultural development? I'm especially looking for something with a strong...

29 June 2020 3,684 4 View

How to restaure all may publications automatically?

64 of my publications , have been removed from my profile . The reason is that i didn't confirm my authorship. That is wrong. I usually confirm my authorship. I need a help to restaure...

21 January 2020 8,865 4 View

What could be the most interesting research topic in the field of education?

I am about to write research on education. What can you suggest as a research topic that can make better the educational system, approach or methodology, program and instruction, and even facilities?

28 November 2019 1,991 2 View

Psychic abilities as a serious subject of study in this emerging period of quantum physics, why or why not?

Exploration of various psychic abilities seemed had reached a dead end with the deterministic Newtonian physics. Now, with quantum physics gaining much progression, should psychic abilities be...

25 August 2019 840 2 View

Do geopolymerization or alkaline activation of an aluminosilicate can display an endothermic behavior?

It is well known that the geopolymerization is an exothermic reaction. But, the measurement of heat flow at different temperatures using Isothermal calorimeter has displayed the decrease of the...

03 May 2019 3,827 1 View

(Li-S batteries) High sulfur loading using Doctor Blade technique?

I am trying to increase the sulfur loading of my cathode using the doctor blade technique. I have no problem to achieve loadings of 1-2 mg/cm2, but i am not able to reach higher...

02 January 2019 6,827 2 View

Does transposable elements in humans carry human genes?

In case of humans does any transposon type move genes that code for such human proteins that have functions? I've read at several sources that they transcribe the proteins needed for the...

14 September 2018 2,698 4 View

How you solve a problem/task like cooking a pizza or exit a room etc.? How you know what is the next step which is lead to the solution?

How do you know that from the almost infinite action (toolbox), which action you need to choose (which is A , B etc..) to get the next step within the progress toward the target. (Starting point...

30 July 2018 2,763 8 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

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02 March 2021 3,060 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View