Which among the 3 major RNA molecules performs the most important function during protein synthesis?

Some proteins or peptides are synthesized non-ribosomally. Do we really need the presence of all 3 RNA molecules in protein synthesis

01 February 2019 4,423 4 View

Hello. Please can anyone provide me with soil fertility parameter limits from any country?

We have analyzed some soil samples for total phosphorus, total nitrogen, water content, pH and soil organic carbon. For meaningful inferences and conclusions, these data have to be compared to...

04 May 2018 5,764 4 View

Dear colleagues, is there really no way the equation for determination of enzyme activity can be standardized?

I have seen several equations in literature presented for the determination of enzyme activity. I have seen Actvity = Va-Vb x M x 1000 all divided by t x Vc; where Va =Volume (mL) of reagent in...

10 November 2017 2,384 3 View

Hi. Please is it possible for human breast milk to contain microorganisms?

I read an article and actually met someone who suggested that human breast milk contains microorganisms and could be a source of novel bacteria

03 April 2017 1,255 8 View

Please can anyone refer me to a site that can give me a step-by-step procedure for artificial neural network analysis in Matlab?

01 January 1970 1,223 7 View

What form of phosphorus is most suitable for incorporation into microbial medium for metal toxicity studies?

01 January 1970 3,193 1 View

What statistical software would be recommended for artificial neural network modeling for an optimization process?

I am trying to compare response surface models with those of artificial networks as current researches in this area tend to favour neural network modeling.

01 January 1970 204 10 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

What kind of steel moulds are used for hot pressing metal powders?

My main concern is that the metal powders should not weld to the mold surface and I should easily be able to eject out the pressed samples. I am looking for alternative for graphite moulds. The...

22 February 2021 2,965 4 View

How can we add reinforced thermoplastics with granules in injection molding machine?

I need to make an injection molding machine in which short fibers are to be introduced. Short fiber length and material properties are to be determined and also the material strength are to be...

22 February 2021 4,290 1 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View