I have had challenges with different forms of phosphorus binding to several metals, immobilizing them and reducing their toxicities to a gram negative bacterium. The most common solution is increasing metal concentration which results in wrong conclusions regarding metal minimum inhibitory concentrations and other useful indices of toxicity. The problem with phosphorus is its general requirement for cellular metabolism particularly in the synthesis of nucleic acids and energy storage, and therefore has to be present in microbial media.
Haribabu Gogula
I think that sodium phosphate may be suitable for your study because of the stadium ion levels with in the cell remain constant .
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