Dear Maurice, you can isolate  bacteria from human breast milk including beneficial

lactic acid bacteria. They can also show probiotic characteristics.

Best regards.

Dear Maurice

 Pls. find the attached files and the references, good luck

Hoping this will be helpful to your project

Regards

Prof. Houda Kawas

https://www.sciencedaily.com/releases/2013/01/130104083103.htm

https://blogs.scientificamerican.com/lab-rat/the-bacteria-in-breast-milk/

http://www.livescience.com/25984-breast-milk-bacteria.html

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2861563/

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4414019/

http://www.medicalnewstoday.com/articles/254758.php

http://michelsonmedical.org/2014/11/07/breast-milk-beneficial-bacteria/

http://www.livescience.com/25984-breast-milk-bacteria.html

Thank you very much my friends. I was under a heavy impression that the mammary gland and its fluid were gnotobiotic. Just never had to look this up.

You most welcome

Houda

Dear Maurice, this book is a good reference for you.

Prebiotics and Probiotics in Human Milk Origins and Functions of

Milk-Borne Oligosaccharides and Bacteria. 

Michelle K. McGuire

Mark A. McGuire

Lars Bode

Academic Press. 2017

Dear Maurice,

Please a couple of links below, FYI. I would suggest reading some papers by MC Collado and co-authors. Hope it is helpful.

Best wishes,

Daniel

https://www.ncbi.nlm.nih.gov/pubmed/27790209

https://www.ncbi.nlm.nih.gov/pubmed/28301837

https://www.ncbi.nlm.nih.gov/pubmed/27159888

Yes, the breast milk has prebiotics. Hopefully this paper below answer your question.

https://www.ncbi.nlm.nih.gov/pubmed/28744259