How to perform PCoA in R with given UniFrac distance matrix?

I have a distance matrix (weighted UniFrac distances) and I want to perform PCoA Analysis in R. I tried: beta

26 September 2019 5,762 2 View

Recommender system for choosing university electives - what recommendation algorithm should we use?

Hi, We want to make a recommender system for students who are in the process of choosing electives at their University. So basically what we want to figure out is: If student x has chosen e.g....

08 December 2015 8,531 11 View

Has anyone experience with fractionation of broadly distributed polybenzyl acrylate via SEC and a fraction collector?

I'm trying to fractionate polybenzyl acrylate for quite a while to get narrowly distributed fractions of different molecular weight, but I can't get a sufficient separation or I have high...

16 March 2015 1,225 3 View

What statistical analysis should I use for Likert-Scale data?

I am trying to determine whether there is an association between time spent online and reported problems. I have divided my participants into two groups: the low user group and the high user...

28 March 2014 3,449 84 View

Which functions are related to different brain rhythms?

Can you suggest good material about the functions of different brain waves (delta, theta, alpha, beta)? In which processes different rhythms are involved? I have found some material of course, but...

09 July 2013 7,953 18 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View