Which concentration of the caspase-1 inhibitor Z-WEHD-FMK is suitable for experiments with primary macropages (BMDM, C57BL6/J)?

07 July 2022 1 4K Report

Hello!

We would like to treat BMDM with the caspase-1 inhibitor Z-WEHD-FMK before bacterial infection. I only found two publications suggesting either 20 or 100 µM for primary macrophages. Of course, titrating the concetration is one option. However, I will be very grateful if someone would share their experience and would recommend a concetration that worked for them. Thank you!

Best regards, Katrin

Katrin Ehrhardt

Maybe someone will be interested in this question in the future, so I share our experiences. We did a titration of 20, 50, and 100 µM Z-WEHD-FMK starting treatment 1 h prior infection of the BMDM. We see a dose dependent reduction in the IL-1beta meaured by ELISA 1 day post Salmonella infection. We will use 50 µM for our experiments.

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