Databases for cell lines?

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How do creative and critical thinking affects the development of 21st-century competencies?

28 January 2024 8,078 5 View

What is different to calculate residual effect in path analysis by formula: Residual effect = 1- R2 or Residual effect =√(1-R2)?

08 January 2024 2,888 2 View

What is the influence of the satellite's attitude on the accuracy of the instantaneous range?

Assume I have the coordinates of satellite xs (t) and ground station xg (t) in the International Terrestrial Reference Frame (ITRF). Also, the attitude of satellites in the form of quaternions is...

10 December 2023 8,820 0 View

What is the minimum number of C2C12 cells for Western blot?

I would like to observe myosin heavy chain and myosin expressions in C2C12 3D samples. What is the least amount of cells required for Western blot? If I start with 10000 cells, will the results be...

23 October 2023 4,746 0 View

In focus reduction neutralization assays, there is a lot of variation between the virus phenotypes between each well. Can anyone explain this?

In focus reduction neutralization assays, we apply a virus and serum mixture to a monolayer of cells. After infection, incubation, fixing, and staining we count the number of foci (formed from...

07 June 2023 4,211 1 View

How can the divergence between the transmitting laser and the retroreflectors be minimized in Satellite Laser Ranging (SLR)?

The general characteristics of Satellite Laser Ranging (SLR): The photons returning are usually fewer because the transmitting laser and retroreflectors both have a divergence. This means that the...

10 May 2023 9,289 10 View

Does stakeholder pressure influence the extent of corporate water disclosure in water-sensitive industries?

29 April 2023 6,224 1 View

How can I download Cell Profiler Pipeline for C2C12 myoblast cell differentiation?

For analysis of C2C12 myoblast cell differentiation in Cell Profiler Software.To assess Myosin Heavy Chain expression in differentiated C2C12 cells.

10 April 2023 4,523 2 View

Is the frequency drift value constant, linear, or nonlinear variation in the case of an atomic clock?

In an atomic clock: instability in the oscillator and environmental changes can cause frequency drift, although it is often hard to identify between drift and oscillator aging. So, is the...

16 March 2023 2,774 4 View

Shall I combine my analysis and discussion for my dissertation?

Hi guys, I wanted to ask a question about my dissertation. It is an IPA study and I am wondering if I should combine the analysis and discussion section or leave them separate. Most journals I...

08 March 2023 2,490 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View