Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

Are the molecular biology reagents of HiMedia ( kits,pcr and electrophoresis products) as good as products of NEB, Sigma and Invitrogen?

HiMedia seems to be cheaper than the rest.Anybody who has used it for molecular biology experiments, how is the quality?

26 September 2019 7,010 3 View

Annealing temperature in RT PCR - Positive control differes from target gene?

The primer annealing temperature , after the gradient PCR analysis, for the target gene differs from the optimal annealing temperature in housekeeping gene (positive control). What to do ? The...

02 July 2019 810 3 View

What statistical test can i use on my data?

I am researching the relationship between animal biodiversity and physics-chemical properties of a clean and polluted burrow dyke. I have the species abundance for both burrow dykes, as well as...

26 June 2019 569 2 View

Segregation coefficient of self-compacting concrete and small superplasticizer overlaps?

What do you think how important, for segregation coefficient of SCC, could be very small superplasticizer overlaps during the process of making SCC? I made some observations in my work: Some...

04 May 2019 6,951 4 View

What policies may bring about sustainable reform and development?

Social disparities rise amid volatile economic and political times today. What may be constructive efforts in policy form to start resolving issues that cause such state of affairs?

16 March 2019 1,086 4 View

How can I improved the power (accuracy) of my Deep Learning Model?

Hey everyone, me and my colleagues are programming a Deep Learning Model with tensorflow. Our goal is it to predict chemical toxicity. Our input are chemical drugs in SMILE-Strucuture. So, how...

07 June 2018 7,920 3 View

What are uses of waste in geotechnical engineering?

waste use in improving soil engineering specification such as disaster waste, cement waste and others

02 August 2017 8,663 16 View

Dummy/ real data Request

02 July 2017 5,438 2 View

Do you know a scale for entrepreneurial learning?

Looking for a scale for entrepreneurial learning types (individual vs. collective learning, exploratory vs. exploitative, intuitive vs. sense making learning

20 September 2016 4,647 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View