Hi darwin software do not use pic values. You must use dominant or codominant score file as input file of darwin. If you need help i can send you sample input file. 

Dear Ibrahim

Thanks for the answer. The input format for Darwin is not clear to me. I have for each primer different number of bands in 5 varieties.  How to make or prepare the format that will suit the Darwin software? Your input file would be most probably very helpful;; I would appreciate if you send it to me.. :)

I will send you the file.

Ibrahim çelik can you please also send me sample input file for Darwin? Thanks!

Dear Ibrahim çelik, can you also send me sample input file for Darwin? Thanks!

Dear All,

We have analysed our germplasm with GBS data. Now, I have filter up to 36000 SNPs with MAF = 0.3 and missing data = 0. I want to construct a phylogeny tree for it. I want help for it.

@Abd Ullah Darwin software ıs not suitable for gbs, it ıs better to use Tassel software.

Dear Ibrahim,

How can I prepared my data for tassel software? I face problem for AMOVA, structure and phylogenetic analysis. Your help in this regards will be highly appreciated. When you get a chance please guide me.

Thanks in advance

I have drawn a tree with genetic distance produce through GenAlex through R.

Hi

you can download input file (demo.txt) and other files generated during calculations. (file contains dominant data)

https://drive.google.com/open?id=1ycMcWGQHKs1mlW-UOQjcPPLrfO7Nmw2a

For help you can contact me:

[email protected]