Jasmin Sutkovic

6 Questions 15 Answers 0 Followers

Questions related from Jasmin Sutkovic

Annealing temperature in RT PCR - Positive control differes from target gene?

The primer annealing temperature , after the gradient PCR analysis, for the target gene differs from the optimal annealing temperature in housekeeping gene (positive control). What to do ? The...

03 July 2019 949 3 View

Why do I get UREA PAGE GEL smeared bands?

For the past two months I have been trying to analyse my AFLP regions on a 6% UREA PAGE GEL. The sample are selectively amplified with 4 set of primers.I have tried to optimize the protocols for...

03 December 2015 10,096 13 View

Any advice on denaturing urea PAGE gel electrophoresis staining?

Dear All, After a successful amplification PCR I am about to visualize my AFLP fragments. Most of the protocols suggests UREA PAGE. The protocol i used ...

22 October 2015 6,836 4 View

Why does my diagnosis restriction digest not work when PCR verification of my insert works?

Dear all, I have transformed my Ecoli with a clone of size (14.3kb). Plasmid alone is about 13.9kb and insert of size 432bp. In 1:3 ratio I got around 200 colonies, negative control plate (only...

25 September 2014 8,686 4 View

Darwin Software - pylogenetic tree construction based on allel number ?

Respected, Does anyone know how to construct a phylogenetic tree base on (PIC) and heterozygosity (H) values calculated for each SSR primer using Darwin software ? The PIC and heterozygosity...

01 January 1970 465 10 View

Why SSR DNA markers produce bands that are not the same intensity ?

One of my colleagues questions the results of the SSR analysis I have recently performed. With primer AMS12, we obtained 8 different bands, ranging from 200bp to 600bp. He is insisting that on...

01 January 1970 9,831 5 View