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Questions related from Jasmin Sutkovic
The primer annealing temperature , after the gradient PCR analysis, for the target gene differs from the optimal annealing temperature in housekeeping gene (positive control). What to do ? The...
03 July 2019 945 3 View
For the past two months I have been trying to analyse my AFLP regions on a 6% UREA PAGE GEL. The sample are selectively amplified with 4 set of primers.I have tried to optimize the protocols for...
03 December 2015 10,095 13 View
Dear All, After a successful amplification PCR I am about to visualize my AFLP fragments. Most of the protocols suggests UREA PAGE. The protocol i used ...
22 October 2015 6,833 4 View
Dear all, I have transformed my Ecoli with a clone of size (14.3kb). Plasmid alone is about 13.9kb and insert of size 432bp. In 1:3 ratio I got around 200 colonies, negative control plate (only...
25 September 2014 8,684 4 View
Respected, Does anyone know how to construct a phylogenetic tree base on (PIC) and heterozygosity (H) values calculated for each SSR primer using Darwin software ? The PIC and heterozygosity...
01 January 1970 463 10 View
One of my colleagues questions the results of the SSR analysis I have recently performed. With primer AMS12, we obtained 8 different bands, ranging from 200bp to 600bp. He is insisting that on...
01 January 1970 9,829 5 View