I want to establish cytotoxicity assays for some anti-cancer inhibitors.
I have problems to get 96-well plates immediately. The use of 48-well plate is it ok; with rising only the volume and cell density in each well ?
The other assays such Clonogenic or Colony Formation could be used as alternative or complementary to standard method ?
Ali Athab Al-Kinani
Hi Mohamed,
That should be fine, you can use the 48 well plate. However, as you mentioned, your seeding density will have to change to match the seeding density you use in 96 well plate.
For the assays you mentioned, they are accurate but may be a time Consuming. You can use them as complementary to the cytotoxic assay.
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Mohammed Najim Abed
That is fine just check the difference in surface area between 96 and 48 well plates and seed accordingly, the use your cell growth inhibition assay to find the IC50
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