12 Questions 13 Answers 0 Followers
Questions related from Mohamed Lahmadi
I've cultured cells "cell lines" (stored for a long period in liquid nitrogen). I have a problem with cell attachment, as most cells are still in suspension and some form spheroids. The cells tend...
15 August 2024 2,754 3 View
I've cultured cells (cell lines) "stored for a long period in liquid nitrogen". In addition to low viability, I have a problem with cell attachment to the culture flask (most cells are still in...
26 August 2023 6,367 6 View
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26 August 2023 5,278 6 View
Sanger sequencing method is less sensitive and maybe not suitable for the detection of mutations in ctDNA. However, In case to detect mutations in some samples (ctDNA) with sanger seq; mutant...
08 February 2021 6,099 3 View
It is feasible to freezing lymphocytes and monocytes, isolated from the same blood samples, after one passage? - Ficoll density gradient centrifugation - to wash lymphocytes and culture them in a...
29 January 2021 9,966 3 View
Is there any problem if I storage PCR products after purification (from gel or PCR reaction) so that would be used for sequencing (sanger) for less than one month at 4° or -20°C ?
07 February 2019 9,746 4 View
I have isolated total RNA from FFPE tissues but the concentration is very low (1-2 ng/uL), the amount of 2-5 ng in reaction would work with the analysis of gene expression using sybrgreen in qRT-PCR ?
29 January 2019 2,858 4 View
We are going to purchase qPCR instrument to our new genomic platform; Which the better choice regarding specifications, medium throughput applications and price between this instruments : qPCR...
21 February 2018 2,968 3 View
I want to establish cytotoxicity assays for some anti-cancer inhibitors. I have problems to get 96-well plates immediately. The use of 48-well plate is it ok; with rising only the volume and cell...
02 December 2017 1,529 3 View
I need to preserve DAN RNA samples for shipping via travel, for a period of 6 to 12 hours. How to storage and transport them in RT or other packaging methods ? and what is the protocl of freeze...
07 September 2016 9,010 4 View
I need a detailed protocol for establish a primary cell culture for lung cancer cells. Is there a commercial kit for this purpose ?
23 March 2016 7,796 4 View
If I get a fresh tissue just after surgery. Could I freeze the tissue and conserve it for ulterior use? Or, do I have to use a protocol of conservation with IRPM & Antibiotics and make the...
04 March 2015 5,233 3 View
