Hi,

When designing my CRISPR guide RNAs, to knockout regions of my gene of interest's promoter, I used the CRISPR-P software (http://crispr.hzau.edu.cn/cgi-bin/CRISPR2/CRISPR). However, where I wanted my knockout to be, those sgRNAs had high off target sites (around 40). What would my CRISPR efficiency be ?? So far I genotyped only 10 plants that were hygromycin resistant (and therefore were transformed), and they all did not have the knockout, but that number is pretty low so maybe thats why I haven't seen any yet. I'm scared that I won't get any at all if those off target sites are too high! Any help is greatly appreciated ! Thanks!