Western blot with very acidic proteins, any suggestions?

I am trying to do a Western Blot to detect acidic proteins (~30kDa) that have a PI of ~3.5, due to the presence of Asp and Glu repeats. The proteins are about 80% pure. After I ran them on...

24 January 2021 7,226 3 View

Problems with allele speration by cloning in pKS plasmid?

I am trying to do the allelic separation of a ~8 kb region using cloning strategy with pKS plasmid. However, when I analyze the result of the colony PCR sequencing It seems that both alleles were...

14 December 2020 5,829 2 View

At what concentration does hydrogen peroxide inhibit enzyme activity?

I am trying to measure GUS expression, and was wondering if anyone knew what concentration hydrogen peroxide inhibits the GUS protein??? Thanks ! Any help is appreciated!

23 November 2020 4,101 3 View

Does anyone know a Python script bank to analyze data from thermal images / thermographs?

script, phyton and thermography

13 September 2020 3,951 3 View

Management Innovation and Bahavior economic?

Hello guys! I'm looking for studies that address behavioral economics in Management innovation. Especially regarding the decision-making process.

13 September 2020 8,687 3 View

How to quantify and stratify the volume and specific surface area of submicro-, micro-, meso- and macropores in a porous solid?

Is it possible to quantify and stratify the kinds of pores (volume and specific surface area)? Only N2 physisorption is enough? Is there another technique to help this? Physically are there other...

04 August 2020 9,175 3 View

Is possible connect infrared camera - Flir One Pro 3td generation in windows or linux, to capture images and videos?

I found in internet some drivers and softwares (flironecontrol and others), If anyone knows about more softwares, please share.

29 June 2020 6,641 2 View

What else reduces NBT (nitroblue tetrazolium) to formazan?

I need to produce formazan to make a standard curve, (as in this paper:Quantitative Determination of Superoxide in Plant Leaves Usi... ) The reaction requires riboflavin and methionine,...

05 March 2020 6,403 3 View

Dear colleagues, please lmn what volume do you use for intranasal instillation in rats?

I am in doubt as to the best volume for intranasal instillation in rats, what volume ensures the best distribution of the particles?

20 February 2019 2,920 1 View

For CRISPR, does it matter if the guide RNA is looking for the antisense or sense strand?

So, I've used the CRISPR P 2.0 software to look up guide RNAs to use to delete a region in my gene's promoter. Past members of our lab were using one guide RNA taken straight from that website to...

01 November 2018 1,756 2 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How Can I bring as much common elements in steel as possible to calculate stable phase(s) in OpenCalphad?

As I was tweaking step-by-step OpenCalphad6 Macros step1.ocm involving phase and thermal property calculation of a High-speed steel, I could not incorporate more than 6 elements in the steel...

01 March 2021 8,840 1 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

What is the error of the Txy diagram?

Hi, I know the attached Txy diagram has an error but can't figure it out. Could you please look at the diagrams and explain it to me?

23 February 2021 4,239 1 View

How the values of errors can be calculated for thermodynamic data?

I have calculated thermodynamic parameters for surfactant solutions in aqueous and mixed alcoholic solvents. I want to calculate the value of errors in the data and represent it in the table along...

22 February 2021 239 2 View

How to get/count refractive index in gasses?

20 February 2021 8,202 6 View

How to make Fast Green FCF from powder?

Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...

07 February 2021 1,671 2 View

How could a transcriptional factor purified as inclusion bodies (by using urea) exhibit a ratio 260/280>1?

I am working with a very unstable transcriptional factor. I use DNAse in the lysis buffer and I purify it as inclusion bodies, by using urea and 1M NaCl. After purification, I buffer exchange the...

05 February 2021 2,530 3 View

Which is the best software for the simulation of tribological properties of materials?

I want to simulate the tribological properties of solids, liquids, and fluids, please suggest good software.

02 February 2021 3,947 3 View