Hello all, what I'm wondering is can CRISPR-Cas9 induce different mutations in the same targeted cell line or would we expect to see the same mutation?

I have a knockout myoblast cell line which in which two particular exons were targeted with CRISPR-Cas9 when creating it. this cell line was sequenced (Sanger) to determine the type of mutation. The DNA obtained from the cells was PCR amplified with the primers for that Exon, and then used to transform bacteria cells in order to isolate the two alleles (TOPO-TA Cloning).

when analysing the sequenced data against the WT exon I noticed that some samples had the same type of mutation while others were WT (therefore the original cell line must be aheterozygous for that mutation). However some other samples also had a mutation but a different one. So is this possible with the CRISPR-Cas9 process? for different types of mutations to be introduced into the cell line? Or would we expect to see only the same type of mt, whether that be a deletion or a point mutation or whatever? Thank you kindly for your help!