T-test and Mann-Whitney U test on groups with different sample sizes and variances?

Hello I have 2 groups with different sample sizes of (experts: 5 to 8) and (Users: 28 to 35) answering 21 questions on a 7 points Likert scale about the importance of some dimensions for...

31 January 2021 3,885 7 View

What are the types of optimization problems that can't be tackled by meta-heuristic and heuristic algorithms?

Any decision-making problem when precisely formulated within the framework of mathematics is posed as an optimization problem. There are so many ways, in fact, I think infinitely many ways one can...

06 September 2020 9,415 14 View

Is it possible to reduce tensile strength and increase compressive strength by adding any admixture to concrete?

Hello, I want to know is it possible that the compressive strength of concrete increase by reducing tensile strength? if yes, how we can explanation and justification this issue?

30 April 2020 4,489 7 View

What are the plans for exams and assessments of universities during corona virus pandemy?

What alternative ways universities use for academic assesment of students?

29 April 2020 3,091 1 View

Is there a specific way to discretize a continuous model to any arbitrary multi DOF system and extract the mode shape matrix in abaqus?

27 April 2020 239 3 View

What is best sampling method for cross sectional studies?

I want to research on liver disease patients behaviours and habits in shiraz city. How can I calculate sample size accurately? Thanks

23 March 2020 9,974 5 View

Anyone in kolkata working of LNCaP prostate cells?

I am working with prostate cancer cells, i want to know who is presently working with PCa cell lines in viro and in vivo in kolkata.any leeds will of great help.

07 March 2020 7,831 1 View

How can I find the IDT point of the following TGA curve which is related to a clay soil?

The attached file shows the TGA curve of a clay soil. Can anyone help me how to recognize the initial decomposition temperature (IDT) from this curve?

06 February 2020 6,944 3 View

How to isolate dorsal root ganglia in rat at lumber region?

For pathological investigation of sensory and motor nerves in lumber region of rat how to isolate and preserve

04 November 2019 8,974 1 View

What's the procedure for validation test of a new design of product (Say Heat Exchanger)?

after 3D design of all components of the Heat Exchanger geometry we do assembly and finalization of the geometry, in CFD analysis we change the boundary conditions and it refers us to the...

27 October 2019 9,095 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View