Rajesh Pal

10 Questions 82 Answers 0 Followers

Questions related from Rajesh Pal

Is there any way to find the biological correlation between miRNA and genes?

We are trying to find the biological correlation between miRNA and certain genes. We found the general correlation through IPA (Ingenuity Pathway Analysis). Thank you for your inputs!

17 June 2019 4,248 5 View

Please suggest how should i approach for removal of UMI sequence?

I have mi-RNA sequencing data, where my 3 prime adapter is attached with 12 Nucleotide UMI Sequence (in random manner) along with Reverse Transcriptase primer and Universal Adapter Overall...

14 January 2019 5,819 4 View

Which software do you use for UMI removal?

I have mRNA sequence, from which i need to clip the UMI tag. Kindly suggest.

21 December 2018 1,247 2 View

Transcription factor binding and expression?

I have a transcription factor protein X and X has a co-activator protein Y. XY bind to the DNA and downstream expression of proteins occur. Now I block the activity of X through inhibitor but dont...

18 August 2018 9,723 5 View

How to carry out membrane protein and peptide simulations?

I want to study the dynamics of ABC transporters along with a peptide molecule which in physiological conditions passes through the transporter. I am clueless how to set the peptide molecule in to...

05 June 2018 3,178 4 View

What is the difference between RMSD and RMS value?

I know what RMSD value is but i often encounter RMS in pymol as well as in certain docking programs. I tried to search the term online but didn't find any satisfying answer.

28 November 2017 1,550 6 View

Has anybody mastered the process of plasmid isolation manually?

I have been isolating plasmid manually for the last few weeks using PCI method but i never got results. tried tons of protocols available online but i dont get it where i am going wrong. Even if i...

03 April 2017 6,219 4 View

Why isn't there more research happening on Phage therapy ?

we are already facing multi drug resistant bacteria and people are dying everyday. why there is little research happening on this field all over the world ovbsly leaving Russia, poland etc. Why...

13 March 2017 8,315 1 View

When a drug is administered does it require a certain amount of protein to show its effects.?

Recently i asked this question to a pHD student who has targeted a protein which is involved in splicing, and overexpression of it has been linked to cancer . Now, i questioned him that, the...

09 March 2017 1,706 2 View

SDF file split?

how can i split the zinc SDF file?pls explain step by step procedure

16 April 2016 5,876 1 View