Could you tell me the composition of the wash buffer in RNA extraction (Silica Magnetic Bead-based) and its proportions?

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How to remove the salt and get purified DNA in Silica Magnetic bead-based Nucleic acid extraction?

I am doing nucleic acid extraction (manual silica magnetic bead-based) from the serum sample. After the extraction, I have taken the OD 260/280 in UV spec. There is no protein contamination. But...

04 November 2020 1,061 2 View

Best book for Fluid Structure Interaction?

Practical methods along with the basic behind Fluid Structure Interaction.

11 June 2020 9,743 9 View

Most of the Research papers are found more theoretical and previous work but only few paragraph spent in experiment and result , Why ?

My research is going on Traffic analysis , Prediction and Intelligent Transport System. I have good concept in theory but I found that most of the papers are not elaborated experiments setup...

17 April 2020 407 12 View

Can I use Phosphate Buffer for Antibody and Fluorescence conjugation?

I am going to use Phosphate Buffer (pH 6.5) is an alternative of MES buffer (pH 6.5) for Antibody and Fluorescent Microspheres conjugation procedure. The literature I am referring to mentions MES...

22 December 2019 794 3 View

Do I need to do a polymerization reaction under a nitrogen atmosphere?

I am polymerizing styrene with Potassium Peroxodi Sulfate and Acrylic acid by an emulsion polymerization method. The literature I am referring to mentions stirring the reagents with a nitrogen...

18 December 2019 8,158 4 View

IoT Challenges with Healthcare?

In want do some work in the security of IoT with Healthcare , please suggest me some area , in which area our scholar can do some work. If possible provide some detail from 2018 and 2019 Dr....

12 July 2019 6,628 3 View

Performance Analysis of Big Data ?

How performance of the Big data can be analysis, which framework should used ? Is Hadoop and Spark Framework are good or other tool should use , is any Source for code or algorithm can find?

01 July 2019 348 3 View

I want to study about antibody antigen binding for chikunguniya and malaria virus? Where can i find relevant material?

Study for Virus diagonesis

23 April 2019 9,210 5 View

Address of Tanusri Mandal is wrong . How to rectify?

Tanusrei Mandal, an author of the paper, entitled "Calcite solubilization by Bacteria: An Novel method of Environmental pollution control ". Published in Geomicrobiology Journal Vol.32, PP.846...

23 January 2018 1,566 3 View

Bandgap Engineering of BTO/bottom electrode/STO (or any suitable substrate) for Photovoltaic Applications.

I am interested in reading some literatures on the Bandgap Engineering of BTO/bottom electrode/GSO (or any substrate), could someone provide me some links of interesting papers on the same?

06 August 2017 3,989 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View