Could you suggest a good control gene for SiRNa in DRG neurons?

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Can anyone recommend an ATG7 antibody in PFA fixed tissue for IHC?

Looking for an antibody for IHC that works in PFA fixed brain tissue.

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Quantitative ISH (non-radioactive)?

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Does anyone have experience with small scale siRNA screening in primary neurons?

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Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

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Mila Angert

Hi Gunnar! Do you mean Housekeeping gene or marker? HK depends on studies, you have to show that HK is not changing.ATF 3 is a good marker gene for DRG.

http://www.genecards.org

A lot of info here

Gunnar Heiko Dirk Poplawski

thank you mila, will try ATF3. :)

Call AB and Roche tech support. They sell a mini kit with different HK genes.b-actin, 18S, cyclophiline,gapdh,HPRT ,PBGD,28S and so on.May be you need to test all of them once to pick a right one.

Jana McCaskill

Hi Gunner. Lamin A/C siRNA was one of the first validating siRNAs and has previously been used in DRG neurons http://www.ncbi.nlm.nih.gov/pubmed/16987960.