Gunnar Heiko Dirk Poplawski

7 Questions 5 Answers 0 Followers

Questions related from Gunnar Heiko Dirk Poplawski

Extracellular GFP expression on neuronal cell adhesion molecule?

Dear all, Has anyone came across an extracellular-tagged cell adhesion molecule. Ideally in the axon and ideally in the growth cone such as L1CAM or NCAM. I would like to tag the extracellular...

03 February 2021 8,067 3 View

Does anyone has experience with too much T2A read-through?

Dear Colleagues, I am expressing two proteins on a polycistronic mRNA separated by the T2A sequence in the mouse brain. I have encountered equal amounts of fusion protein as well as separated...

21 January 2021 7,414 1 View

What are the cheapest streptavidin magnetic beads?

Doing some protein pulldown with biotinylated antibodies. There are now so many alternatives to Dynabeads. Has anybody done a price/quality comparison? Happy new year to everyone :)

02 January 2018 4,213 4 View

Could you suggest a good control gene for SiRNa in DRG neurons?

I am looking for a recommendation for a good control siRNA (housekeeping gene) to confirm knockdown in DRG neurons? GAPDH kills the DRGs Thanks a lot gunnar

09 December 2014 8,531 5 View

Can anyone recommend an ATG7 antibody in PFA fixed tissue for IHC?

Looking for an antibody for IHC that works in PFA fixed brain tissue.

10 May 2014 7,265 1 View

Quantitative ISH (non-radioactive)?

In order to validate mRNA expression in the mouse brain following RNAseq, I would like to do quantitative ISH. If you have any recommendations of which technique to use or company suggestions,...

29 January 2014 5,845 2 View

Does anyone have experience with small scale siRNA screening in primary neurons?

I am planing to perform a follow up screen to RNAseq most likely in adult DRG neurons or hIPSC derived neurons. I am looking at the flexiplate siRNAs from Qiagen (100pmol/23USD) or Mission...

09 January 2014 5,568 5 View