Lyse-n-go PCR reagent discontinued....alternatives? ingredients?

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No title

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Charles D Anderson

I found this online a moment ago lookng for the ingredents from the MSDS for Lyse-n-go:

Article Epitope Tagging of Endogenous Proteins for Genome-Wide Chrom...

3.Lyse-N-Go is a reagent from Pierce that is useful for the rapid, inexpensive production of template DNA from cells. Such templates have been used successfully for a number of PCR reactions in which products of up to 5 kb have been amplified robustly. However, Qiagen genomic DNA prep kit is an expensive alternative to produce better quality DNA.

Or something like:

SDS-NaOH lysis buffer

0.25% SDS (sodium dodecyl sulfate)

0.05 N NaOH

Best Wishes!

Pablo Enrique Grijalba

Try with the easy extraction from

Collado-Romero M., Mercado-Blanco J. , Olivares-García C., Valverde-Corredor A., and Jiménez-Díaz R.M. 2006. Molecular Variability Within and Among Verticillium dahliae Vegetative Compatibility Groups Determined by Fluorescent Amplified Fragment Length Polymorphism and Polymerase Chain Reaction Markers. Phytopathology: 96 (5) 485-495

Pablo