What are the cheapest streptavidin magnetic beads?

Doing some protein pulldown with biotinylated antibodies. There are now so many alternatives to Dynabeads. Has anybody done a price/quality comparison? Happy new year to everyone :)

01 January 2018 4,087 4 View

Lyse-n-go PCR reagent discontinued....alternatives? ingredients?

Dear all! Unfortunately the Lyse-n-go direct PCR reagent is not more available from Thermo. Does anybody has an idea for an alternative? Or knows the "ingredients"?. We aim to lyse biofilms on...

26 November 2017 5,874 2 View

Studies estimating the total biomass of (flying) insects?

I am looking for studies which estimate the total biomass of insects in kg/area depending on habitat types. It would also be important to know, which proportion of these enter the air. Thank you...

21 November 2016 525 3 View

How to make permanent microscope slides of arthropod material after immersion in DNA extraction buffer?

When I slide-mount Diptera specimens, I typically macerate them for ca 24% at room temperature in 10% KOH (pH between 12.5 and 12.8). This removes a lot of muscle tissue and pigments, and make it...

11 January 2016 7,295 5 View

Can anyone recommend an ATG7 antibody in PFA fixed tissue for IHC?

Looking for an antibody for IHC that works in PFA fixed brain tissue.

09 May 2014 7,140 1 View

Quantitative ISH (non-radioactive)?

In order to validate mRNA expression in the mouse brain following RNAseq, I would like to do quantitative ISH. If you have any recommendations of which technique to use or company suggestions,...

28 January 2014 5,733 2 View

Does anyone have experience with small scale siRNA screening in primary neurons?

I am planing to perform a follow up screen to RNAseq most likely in adult DRG neurons or hIPSC derived neurons. I am looking at the flexiplate siRNAs from Qiagen (100pmol/23USD) or Mission...

08 January 2014 5,450 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View