If any of you could suggest me a bioreactor for bone marrow derived MSC culture I would appreciate it. At least what should be the criteriaons? or any brand?
The Mobius CellReady 3L, UniVessel SU 2L, Sartorius Stedim's top-driven BIOSTAT Cultibag STR 50L, Wave Bioreactor 2/10 (GE Healthcare) and you can find more information in this book chapter:
As a co-auther of the book chapter, I appreciate sharing it - thank you Selim.
If I may add: the suitability of a specific bioreactor depends on many factors related to both the characteristics of your cells (source, pre-treatment, etc.) and the desired application. Is the primary focus on small-scale/screening experiments, or should an existing process be scaled-up? So, what is the maximum working volume? For very small scale experiments, even 2D cultivation systems (T-flask, Cellstack etc.) may be sufficient - even though they have several drawbacks. Furthermore, the mentioned bioreactors (all of them are single-use) have been developed for suspension cell cultures and, therefore, microcarriers are typically required for the expansion of the anchorage-dependent MSCs. Numerous studies have demonstrated that the results then not only depend on the bioreactor itself but also on the type of microcarriers, their concentration, seeding density, etc. However, if microcarriers are used, the most important criterion is to achieve homogenous suspension at very low shear stress in order to avoid undesired differentation and/or cell damage/death. Besides the basic engineering characteristics of the individual bioreactors (such as oxygen supply, mixing, shear stress), the user should also consider instrumentation and automation - so it is also important if the bioreactor comes together with a suitable control tower (or if third-party equipment is required), even though this may be not the most important question (but should not be completely neglected).