Hello;
We want to use NK cell for our hematologic oncology patients. I found some papers about it but couldnt be sure. If you could share your protocol or at least address me to a paper please I would appreciate it..
I am looking for protocol for NK isolation, NK expansion and NK quality control tests
Thank you
B.S.
Hi Berrin, Prof Cerwenka´s lab from DKFZ, Germany works extensively on NK cells. Please follow their papers. Miltenyi offers a human NK cell isolation kit and I know that their lab uses that. They use a triple cytokine cocktail for expansion. Please follow their lab papers for anything related to NK cells.
Sustained effector function of IL-12/15/18-preactivated NK cells against established tumors.
Ni J1, Miller M, Stojanovic A, Garbi N, Cerwenka A.
IL-12-producing monocytes and HLA-E control HCMV-driven NKG2C+ NK cell expansion.
Rölle A, Pollmann J, Ewen EM, Le VT, Halenius A, Hengel H, Cerwenka A.
Hope it helps. All the best!
Dear Berrin,
as stated above Miltenyi offers both NK cell isolation and NK cell expansion kits. We frequently isolate human NK cells from peripheral blood using their NK cell isolation kit (negative selection, isolated NK cells are untouched) with 98% purity and 50-70% recovery. We also tried their NK cell expansion kit which actually is a cocktail of cytokines as mentioned above and had good expansion with NK cells in culture for 2 weeks.
I hope this helps, kindest regards
Semih