14 Questions 31 Answers 0 Followers
Questions related from Peter V. Zolotukhin
Dear colleagues, I'm prettey used to preparing chitosan/TPP nanoparticles, but for my further studies, I'll need to disassemble them. Surely I can use enzymatic digestion of chitosan, but I'd...
04 April 2017 1,995 3 View
Dear colleagues, I have a very silly question, but it bothers me a lot. When working on cell viabiity using a hemocytometer, how do you account the cells that were already dead and had detached...
08 August 2016 8,937 5 View
Dear colleagues, I've recently found an interesting paper (PMID 21763280) on the phenotypes of necrotic and apoptotic cells. As seen from Figure 1, cells treated with TNF and CHX undergo either...
08 August 2015 3,384 2 View
Dear colleagues, so the question is in the title... Background: we've spent a few days trying to isolate RNA from an adherent cell culture grown in a 6-well plate. Isolating RNA from various...
03 March 2015 9,390 12 View
Dear colleagues, I see clear difference between the training and testing statistics in MDR, but I don't get (and could not find the answer in a bunch of publications - it is not even discussed...
03 March 2015 427 4 View
Dear colleagues, I've browsed a lot of papers and encountered the fact that when the both primary and metastatic tumors are of interest for researchers and when the xenograft model is involved,...
02 February 2015 3,366 7 View
Dear colleagues, could you please suggest trustworthy custom organic synthesis enterprises according to your experience?
10 October 2014 4,716 0 View
PEG DNA precipitation
08 August 2014 849 2 View
Dear colleagues, should I change the medium after transfecting HepG2 cells with the RNAiMAX reagent for 24 hours? The supplier's protocol does not include this step in the knockdown assay, while...
07 July 2014 4,453 12 View
Please share your knowledge on the faith of oxidized nucleic acid bases. Fast yet extensive PubMed browsing gave no positive results. Most papers deal with processes that lead to free oxidized...
03 March 2014 9,887 1 View
Dear colleagues, how many scrambled siRNAs do you use in case when you either target single gene with two or more siRNAs or target multiple genes with multiple siRNAs?
02 February 2014 4,098 7 View
Do you you have any experience in using this modifications combination to reduce the off-target effects and to promote the antisense strand functional selection?
01 January 2014 3,516 1 View
Which PCR instrument should I choose? For now, I have my doubts between BioRad QX200 and Life Technologies QuantStudio 3D. Maybe neither is good?
12 December 2013 2,505 10 View
Do you use the passive reference dye in DNA-binding-dye type real-time PCR?
08 August 2013 10,013 7 View
