Dear colleagues, could you please suggest trustworthy custom organic synthesis enterprises according to your experience?
Dear colleagues, I'm prettey used to preparing chitosan/TPP nanoparticles, but for my further studies, I'll need to disassemble them. Surely I can use enzymatic digestion of chitosan, but I'd...
03 April 2017 2,022 3 View
Dear colleagues, I have a very silly question, but it bothers me a lot. When working on cell viabiity using a hemocytometer, how do you account the cells that were already dead and had detached...
07 August 2016 8,973 5 View
Dear colleagues, I've recently found an interesting paper (PMID 21763280) on the phenotypes of necrotic and apoptotic cells. As seen from Figure 1, cells treated with TNF and CHX undergo either...
07 August 2015 3,435 2 View
Dear colleagues, so the question is in the title... Background: we've spent a few days trying to isolate RNA from an adherent cell culture grown in a 6-well plate. Isolating RNA from various...
02 March 2015 9,512 12 View
Dear colleagues, I see clear difference between the training and testing statistics in MDR, but I don't get (and could not find the answer in a bunch of publications - it is not even discussed...
02 March 2015 461 4 View
Dear colleagues, I've browsed a lot of papers and encountered the fact that when the both primary and metastatic tumors are of interest for researchers and when the xenograft model is involved,...
01 February 2015 3,402 7 View
PEG DNA precipitation
07 August 2014 882 2 View
Dear colleagues, should I change the medium after transfecting HepG2 cells with the RNAiMAX reagent for 24 hours? The supplier's protocol does not include this step in the knockdown assay, while...
06 July 2014 4,487 12 View
Please share your knowledge on the faith of oxidized nucleic acid bases. Fast yet extensive PubMed browsing gave no positive results. Most papers deal with processes that lead to free oxidized...
02 March 2014 9,923 1 View
Dear colleagues, how many scrambled siRNAs do you use in case when you either target single gene with two or more siRNAs or target multiple genes with multiple siRNAs?
01 February 2014 4,132 7 View
Approximate concentrations are require in compared with the WHO permissible limts
11 August 2024 2,723 1 View
Hey there, As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions...
09 August 2024 5,444 7 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View