Can someone suggest me how to homogenize Artemia nauplii ?

I want to investigate microflora inside of Artemia nauplii, so I want to break the body of Artemia nauplii. what kind of homogenizer machine can I use? Or which method can I use? Thank you so much?

10 November 2016 2,131 4 View

Can someone suggest me the reason why there is no colony on the plate when I perform a plate count of lactic acid bacteria?

I perform a plate count but there is no colony on the plate although they still normally proliferated in the MRSB culture. Please tell me why? Thank you so much.

03 April 2016 6,059 7 View

Could someone help me answer a question about SDS - PAGE?

I did a one dimensional SDS - PAGE. Stacking gel is 5% and Separating Gel is 12%,however, they didn't polimerize. I tried to use progressive concentration of Acrylamide from 12% to 36%, and at 36%...

06 July 2015 1,042 8 View

Could someone help me answer a question about SDS - PAGE?

I did a one dimensional SDS - PAGE. Stacking gel is 5% and Separating Gel is 12%,however, they didn't polimerize. I tried to use progressive concentration of Acrylamide from 12% to 36%, and at 36%...

06 July 2015 892 19 View

Could somebody suggest the method of determining Trichoderma (or other fungi) spore density? Thank you all so much.

I was inoculating Trichoderma in broth culture (submerged inoculation) and I want to determine the spore density? Can somebody help me. Thank you so much!

09 October 2014 2,237 10 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

How to reduce debris with neuron isolation?

Hi there, I'm isolating neurons from PND1 guinea pigs and have noticed a bit of debris in the early days (this is day 4 plated) and a lot of variability between plates. Current protocol is...

14 February 2021 3,692 4 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

How to improve NHBE adhesion after subculture?

I'm trying to subculture NHBE cells from Lonza, however, the cells seem to not adhere to the new flasks after detachment. I've followed Lonza detachment protocol (2 mL/25 cm2 of Trypsin 0.05% and...

10 February 2021 196 1 View

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Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

What are some common oxidants used to oxidize iron sulfur clusters in proteins for techniques like EPR?

I have used potassium ferricyanide (which is likely the most common) extensively in the past, but I am curious to know if there are other oxidants that work well. I need to generate the oxidized...

29 January 2021 9,803 4 View

Papain Enzyme Assay...?

Hi all, I would like to know how can I estimate the activity of the enzyme, papain, which is used in reactions as crude latex extract of papaya. Please share some procedures if anybody having...

26 January 2021 569 4 View