Could somebody suggest me Why we have to use many proteolytic enzymes in order to characterize bacteriocin?

Phúc Tô Đình @Phuc_To_Dinh2

05 May 2015 1 8K Report

Such as trypsin, protease K, Pronase E, Alpha-chymotripsin and pepsin.

How will they effect to bacteriocin?

The purpose is to determine the nature of protein, therefore, What is function of every enzyme for bacteriocin.?.

Thank you

Jaime Antonio Cardozo

I think that these compounds allow splitting the bacteriocin in simple peptides, allowing then to characterize it.

Badges
Science topic

More Phúc Tô Đình's questions See All

Can someone suggest me how to homogenize Artemia nauplii ?

I want to investigate microflora inside of Artemia nauplii, so I want to break the body of Artemia nauplii. what kind of homogenizer machine can I use? Or which method can I use? Thank you so much?

10 November 2016 2,428 4 View

Can someone suggest me the reason why there is no colony on the plate when I perform a plate count of lactic acid bacteria?

I perform a plate count but there is no colony on the plate although they still normally proliferated in the MRSB culture. Please tell me why? Thank you so much.

03 April 2016 6,252 7 View

Could someone help me answer a question about SDS - PAGE?

I did a one dimensional SDS - PAGE. Stacking gel is 5% and Separating Gel is 12%,however, they didn't polimerize. I tried to use progressive concentration of Acrylamide from 12% to 36%, and at 36%...

06 July 2015 1,065 19 View

Could someone help me answer a question about SDS - PAGE?

I did a one dimensional SDS - PAGE. Stacking gel is 5% and Separating Gel is 12%,however, they didn't polimerize. I tried to use progressive concentration of Acrylamide from 12% to 36%, and at 36%...

06 July 2015 1,205 8 View

Could somebody suggest the method of determining Trichoderma (or other fungi) spore density? Thank you all so much.

I was inoculating Trichoderma in broth culture (submerged inoculation) and I want to determine the spore density? Can somebody help me. Thank you so much!

09 October 2014 2,435 10 View

Could someone explain the basics of working with enzymes?

I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...

01 August 2024 5,460 5 View

What are some diseases that are caused by overactivity of enzymes?

I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...

30 July 2024 2,483 5 View

I have two problems: 1) the enzyme is not immobilizing efficiently into the MOF material.. 2) the MOF itself has peak on 400nm by using p-NPA test.?

I am working on carbonic anhydrase immobilization into MOFs. I am facing problems with low enzyme loading.. The other issue is that when using p-NPA activity test to detect the activity of the...

20 July 2024 1,440 3 View

What are the modern topics to work under bacteriocin and fermentation as PhD student?

As PhD student specialized as food and industrial microbiology need to further research on bacteriocin and fermented food.

16 July 2024 7,394 1 View

Can it is possible to find the cleaved sequence when a protein cleaved by a heamaglutanin protease (HA/P) by any bioinformatics tools?

Bioinformatics tools like peptide cutter

15 July 2024 6,453 1 View

ATG in pPICZalfphaA vector: Yes or Not in front of the gene of interest ... ?

Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...

10 July 2024 3,050 5 View

How does the catalase enzyme contribute to the process of photosynthesis in plants?

كيف يساهم انزيم الكاتلاز في عملية التمثيل الضوئي في النباتات؟

09 July 2024 7,512 1 View

What is the role of catalase enzyme in plants?

ماهو دور انزيم الكاتليز في النباتات؟

09 July 2024 5,824 2 View

What is the protocol for enzyme freeze drying?

what is the protocol for enzyme freeze drying?

09 July 2024 6,464 6 View

How do I make 10 cc of enzyme solution with a concentration of 0.2 units/mL?

Hi We have alpha-amylase enzyme that written 5 units /mg protein on its container. How do I make 10 cc of enzyme solution with a concentration of 0.2 units/mL?

08 July 2024 2,285 2 View