I did a one dimensional SDS - PAGE. Stacking gel is 5% and Separating Gel is 12%,however, they didn't polimerize. I tried to use progressive concentration of Acrylamide from 12% to 36%, and at 36% they polimerized but not totally. They were still sticky and dissolved away when they were put in the water or staining solution.

Is gel/Acrylamide broken down?

Thank you so much

  • Similar topics
  • Gels
More Phúc Tô Đình's questions See All
Similar questions and discussions