Can someone suggest me the reason why there is no colony on the plate when I perform a plate count of lactic acid bacteria?

I perform a plate count but there is no colony on the plate although they still normally proliferated in the MRSB culture. Please tell me why? Thank you so much.

03 April 2016 6,059 7 View

Could someone help me answer a question about SDS - PAGE?

I did a one dimensional SDS - PAGE. Stacking gel is 5% and Separating Gel is 12%,however, they didn't polimerize. I tried to use progressive concentration of Acrylamide from 12% to 36%, and at 36%...

06 July 2015 1,042 8 View

Could someone help me answer a question about SDS - PAGE?

I did a one dimensional SDS - PAGE. Stacking gel is 5% and Separating Gel is 12%,however, they didn't polimerize. I tried to use progressive concentration of Acrylamide from 12% to 36%, and at 36%...

06 July 2015 892 19 View

Could somebody suggest me Why we have to use many proteolytic enzymes in order to characterize bacteriocin?

Such as trypsin, protease K, Pronase E, Alpha-chymotripsin and pepsin. How will they effect to bacteriocin? The purpose is to determine the nature of protein, therefore, What is function of every...

04 May 2015 7,362 1 View

Could somebody suggest the method of determining Trichoderma (or other fungi) spore density? Thank you all so much.

I was inoculating Trichoderma in broth culture (submerged inoculation) and I want to determine the spore density? Can somebody help me. Thank you so much!

09 October 2014 2,237 10 View

Any recommendations for methods to analyze elephant space-use (using GPS) and correlating this with changes in NDVI?

For my Masters I am looking into elephant space-use and its effect on vegetation change. I have elephant GPS collar data and was going to use Google Earth Engine to obtain NDVI values. I would...

16 February 2021 7,097 3 View

How do you annotate figures to show significance?

30 January 2021 1,008 3 View

Helicoverpa armigera eggs are not hatching in lab condition, why?

Dear RG friends, I am providing a complete H. armigera diet for larvae and maintaining the eggs in the growth chamber as well as in-room conditions. But since three months, eggs laid from the...

21 January 2021 5,546 32 View

Can't find an ancient zoology paper ?

I'm desperately looking for an old planarian paper which is cited here. Does anyone have an idea how to get hold of it...

21 January 2021 5,957 8 View

Genus name priority from the same publication (Zoology)?

I came across a recent taxonomic work, where two genera were synonymized. Both genera were published in a paper in 1922 by the original author who revised the family at that time. The original...

19 January 2021 1,920 4 View

Which statistical test to use to see if there is a significant difference between the vigilance of bird species at different feeders (in SPSS)?

I have gathered data on bird species visiting 4 hanging feeders in different locations and have calculated the proportion of time they have spent displaying vigilant behaviour while at the feeder....

18 January 2021 9,959 7 View

Which is the most recent and updated classification of Class: Insecta?

Based on the many publications from several decades, we came across many classifications of Insects. Among them which one is more updated and approved classification that can be used for research,...

05 January 2021 3,668 9 View

How can we say a species of insects got extinct?

02 January 2021 4,232 10 View

What was scientist thinking about Bees communication before Karl von Frisch discovery?

In the 1920s, Karl von Frisch pointed out that bees use special dancing patterns. What scientist or people think about bees communication before its discovery.

22 November 2020 1,426 5 View

If anyone can suggest or review manuscript having keywords Riparian zone, River, Species richness, Species evenness, Ornithofauna?

Greetings I am Sachin Kumar, pursuing my Ph.D. in Ornithology, from PAU, Ludhiana. I am working on avian diversity along the River Beas. I had submitted a manuscript to a journal having keywords...

10 November 2020 7,194 6 View