11 Questions 12 Answers 0 Followers
Questions related from Liming Zhang
Why kidney cells are always applied for the replication of viruses? Like MDCK and MDBK for IAV, Vero for HSV, BHK and MA-111 for YFV.
16 March 2019 958 4 View
is there any easy protein-protein interaction prediction tools based with amino acid sequence online? I'll detect the interaction between proteins. I want to view the prediction before trying...
03 December 2018 6,058 1 View
What is the white cloudy precipitation after lysing HEK-293T cells? My lysis buffer is composed of 1%NP-40, 150mMNaCl, 50mMTris, 5mMEDTA. After lysing 30min and centrifuge at 1,2000 *g with 30min,...
13 May 2017 9,063 1 View
I want to use influenza A virus rescued from plasmids to infect MDCK cells. Could polybrene can improve the efficiency of the infection of influenza A virus?
12 May 2017 574 3 View
Could lysate be removed detergent, such as NP-40 and TritonX-100, if we treat the lysate with acetone? And how much i would lose the protein if i do this? Thanks.
10 May 2017 3,974 3 View
To rescure virus, will it be created a influenza A virus if i replace a protein expressed in H9N2 with the counterpart in H7N9? For example, i transfect HA, NA, NS, M, NP, PA, PB1 from H9N2 and...
17 April 2017 3,653 3 View
which detergent could be compatible with mass spectrometry?
08 November 2016 6,274 6 View
Is there any different results between 150mM NaCl and 75mM NaCl lysis buffer if i do a co-IP? I want to find the interaction between my interest protein and the other eight proteins.Last week,i...
28 June 2016 1,971 4 View
Which is the best concentration to generate the stably infected MDCK by Zeocin? The MDCK is infected by lentivirus.
03 January 2016 7,292 1 View
I have been struggling with ligation for long fragment(7kb+11kb).The vector is 11kb and was digested by ASCⅠ(a stick end) and NruⅠ(a blunt end).After digestion,phosphatase was added into the...
08 December 2015 9,129 3 View
For past one month, I have been struggling with a ligation reaction. I have been trying to insert a ~7kb DNA fragment into ~11kb plasmid and both them have been preparation by restriction enzyme...
15 November 2015 3,596 4 View
