How to purify a special lipid, such as cholesterol and phospholipids?

How to purify a special lipid, such as cholesterol and phospholipids? I need to purify the whole cholesterol in the blood to the exclusion of other lipids. Is there any special molecules to purify...

04 May 2019 7,035 4 View

Which laptop configuration should i choose for bioinformatics?

which laptop configuration should i choose for bioinformatics? I'm going to study the bioinformatics. But, the professor told me that my laptop isn't suitable for bioinformatics. Therefore, i...

11 December 2018 6,936 0 View

Can SDS-PAGE detect monomer and polymer?

Can SDS-PAGE detect monomer and polymer? If it can, DTT should be added prior to load in gel?

05 June 2018 3,720 1 View

Which concentration of the Bis could the native page need?

05 June 2017 9,348 3 View

What is the white cloudy precipitation after lysing HEK-293T cells?

What is the white cloudy precipitation after lysing HEK-293T cells? My lysis buffer is composed of 1%NP-40, 150mMNaCl, 50mMTris, 5mMEDTA. After lysing 30min and centrifuge at 1,2000 *g with 30min,...

04 May 2017 375 0 View

Why CHAPS cannot solve in water after shaking?

I added 1 g CHAPS into 100ml water and shook it. However, it didn't become clear. Why? How could i do to obtain a clear 1% CHAPS?

10 November 2016 6,309 0 View

How much concentration of CHAPS can i use in lysis buffer?

10 November 2016 6,497 3 View

Could cells from proliferation express the plasmid which i have transfected?

08 September 2016 8,298 0 View

Could I get a high yield human protein production in MDCK?

04 May 2016 4,596 3 View

Western Blot can not detect the expression of protein, although the green fluorescence is expressed. Why ?

Western Blot can not detect the expression of protein, although the green fluorescence is expressed. Why ? The vector is CMV promoter and the sequence is that eGFP-2A site-Myc-insert. There are...

03 April 2016 2,751 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View