I added 1 g CHAPS into 100ml water and shook it. However, it didn't become clear. Why? How could i do to obtain a clear 1% CHAPS?
How to purify a special lipid, such as cholesterol and phospholipids? I need to purify the whole cholesterol in the blood to the exclusion of other lipids. Is there any special molecules to purify...
04 May 2019 7,140 4 View
which laptop configuration should i choose for bioinformatics? I'm going to study the bioinformatics. But, the professor told me that my laptop isn't suitable for bioinformatics. Therefore, i...
11 December 2018 7,112 0 View
Can SDS-PAGE detect monomer and polymer? If it can, DTT should be added prior to load in gel?
05 June 2018 3,830 1 View
The PI of two proteins are 4.96 and .4.86 and the MW of them are both roughly 15KD. Which concentration of the Bis could the native page need? How about the voltage when running?
05 June 2017 9,422 3 View
What is the white cloudy precipitation after lysing HEK-293T cells? My lysis buffer is composed of 1%NP-40, 150mMNaCl, 50mMTris, 5mMEDTA. After lysing 30min and centrifuge at 1,2000 *g with 30min,...
04 May 2017 469 0 View
My protein is 25KD and its pI is 5.5. How much concentration of my native page could be? The native page could deter the tiny mobility changes? And which is the right running buffer, 0.5*TBE or...
10 November 2016 8,133 0 View
How much concentration of CHAPS can i use in lysis buffer? I would like to purify protein. But only CHAPS is compatible with mass spectrometry.
10 November 2016 6,631 3 View
Could cells from proliferation express the plasmid which i have transfected using Lipofectamine2000? What about the mechanism?
08 September 2016 8,367 0 View
Can I get a high yield human protein production in Madin Darby Canine Kidney(MDCK)? Which factor should be focused on if I choose a cell line to yield human protein production? Why people choose...
04 May 2016 4,670 3 View
Western Blot can not detect the expression of protein, although the green fluorescence is expressed. Why ? The vector is CMV promoter and the sequence is that eGFP-2A site-Myc-insert. There are...
03 April 2016 2,826 4 View
Approximate concentrations are require in compared with the WHO permissible limts
11 August 2024 2,723 1 View
I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...
10 August 2024 4,005 2 View
How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
09 August 2024 3,291 2 View
One can try to generalize the Vandermonde determinant in the following direction: Let $A$ be any symmetric $n$-order square matrix. Consider its powers' diagonal elements $(A^k)_{ii}$ and...
08 August 2024 6,690 1 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Why is nonpoint source pollution potentially more harmful and difference between point and nonpoint sources of water pollution?
01 August 2024 1,180 2 View
How do living organisms play a role in the water cycle and why is nonpoint source pollution potentially more harmful than point source pollution?
01 August 2024 7,061 2 View