13 Questions 11 Answers 0 Followers
Questions related from Liming Zhang
How to purify a special lipid, such as cholesterol and phospholipids? I need to purify the whole cholesterol in the blood to the exclusion of other lipids. Is there any special molecules to purify...
05 May 2019 7,113 4 View
which laptop configuration should i choose for bioinformatics? I'm going to study the bioinformatics. But, the professor told me that my laptop isn't suitable for bioinformatics. Therefore, i...
12 December 2018 7,083 0 View
Can SDS-PAGE detect monomer and polymer? If it can, DTT should be added prior to load in gel?
06 June 2018 3,811 1 View
The PI of two proteins are 4.96 and .4.86 and the MW of them are both roughly 15KD. Which concentration of the Bis could the native page need? How about the voltage when running？
06 June 2017 9,404 3 View
What is the white cloudy precipitation after lysing HEK-293T cells? My lysis buffer is composed of 1%NP-40, 150mMNaCl, 50mMTris, 5mMEDTA. After lysing 30min and centrifuge at 1,2000 *g with 30min,...
05 May 2017 448 0 View
How much concentration of CHAPS can i use in lysis buffer？ I would like to purify protein. But only CHAPS is compatible with mass spectrometry.
11 November 2016 6,573 3 View
I added 1 g CHAPS into 100ml water and shook it. However, it didn't become clear. Why? How could i do to obtain a clear 1% CHAPS?
11 November 2016 6,359 0 View
My protein is 25KD and its pI is 5.5. How much concentration of my native page could be? The native page could deter the tiny mobility changes? And which is the right running buffer, 0.5*TBE or...
11 November 2016 8,117 0 View
Could cells from proliferation express the plasmid which i have transfected using Lipofectamine2000? What about the mechanism?
09 September 2016 8,348 0 View
Can I get a high yield human protein production in Madin Darby Canine Kidney(MDCK)? Which factor should be focused on if I choose a cell line to yield human protein production? Why people choose...
05 May 2016 4,650 3 View
Western Blot can not detect the expression of protein, although the green fluorescence is expressed. Why ? The vector is CMV promoter and the sequence is that eGFP-2A site-Myc-insert. There are...
04 April 2016 2,805 4 View
What the function about Glycine,Tris base and SDS in Western Blot's Running Buffer?
03 March 2016 6,741 4 View
A549 and MDCK which cell line is better if i want to do some research about H9N2 ? And which cell line is easy to be infected by the unconcentrated lentivirus?
12 December 2015 8,687 2 View
