How to purify the isolated RNA if its concentration is very low?

We isolate the RNA from tumor cells which were obtained by laser microdissection, using RNeasy Micro Plus Kit (Qiagen). The concentration of the RNA varies from 7 to 9 ng/ul, RIN - 5.0-8.8,...

02 March 2014 7,343 12 View

Expression microarray: low input of RNA - can anyone help?

I am going to perform whole genome expression profiling of different tumor cells of one breast tumor. I isolate these tumor cells by laser microdissection and extract RNA. The total RNA...

09 October 2013 3,937 12 View

How to get sections from frozen samples abundantly penetrated by fat tissue?

I have a problem with preparation of sections from frozen samples of normal breast tissue containing abundant fat tissue. These samples are not frozen in cryotome (from -20 to -30C). So, I can not...

04 May 2013 2,799 29 View

Homeobox genes, tumor spheroids and architectural patterns

I am looking for data about the expression of homeobox genes, namely HOXC6, in tumor spheroids including spheres formed by cancer stem cells and in architectural patterns of tumor cells in breast...

10 November 2012 9,154 0 View

Cancer stem cells and non-cycling (resting) cells?

I have some troubles with understanding terms "cancer stem cells" and "non-cycling (resting) cells". For example, somebody write that core of tumorspheres (spheroids) contains cancer stem cells,...

10 November 2012 1,169 9 View

Whole transcriptome amplification from cells

Does anybody have experience in whole transcriptome amplification from cells? My usual study includes RNA extraction from cells (n=30-300), isolated from frozen tumors by laser-capture...

10 November 2012 3,853 4 View

What is your experience with in situ PCR?

Do you have any experience in in situ PCR? I think it is good approach to study intratumoral heterogeneity but I do not know nothing about it. In particular, where can I find thermal cycler for in...

09 October 2012 6,323 4 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View