Could chemotherapy influence on miRNA level?

More Evgeny V. Denisov's questions See All

How to purify the isolated RNA if its concentration is very low?

We isolate the RNA from tumor cells which were obtained by laser microdissection, using RNeasy Micro Plus Kit (Qiagen). The concentration of the RNA varies from 7 to 9 ng/ul, RIN - 5.0-8.8,...

02 March 2014 7,401 12 View

Expression microarray: low input of RNA - can anyone help?

I am going to perform whole genome expression profiling of different tumor cells of one breast tumor. I isolate these tumor cells by laser microdissection and extract RNA. The total RNA...

09 October 2013 3,991 12 View

How to get sections from frozen samples abundantly penetrated by fat tissue?

I have a problem with preparation of sections from frozen samples of normal breast tissue containing abundant fat tissue. These samples are not frozen in cryotome (from -20 to -30C). So, I can not...

04 May 2013 2,861 29 View

Homeobox genes, tumor spheroids and architectural patterns

I am looking for data about the expression of homeobox genes, namely HOXC6, in tumor spheroids including spheres formed by cancer stem cells and in architectural patterns of tumor cells in breast...

10 November 2012 9,220 0 View

Whole transcriptome amplification from cells

Does anybody have experience in whole transcriptome amplification from cells? My usual study includes RNA extraction from cells (n=30-300), isolated from frozen tumors by laser-capture...

10 November 2012 3,908 4 View

Cancer stem cells and non-cycling (resting) cells?

I have some troubles with understanding terms "cancer stem cells" and "non-cycling (resting) cells". For example, somebody write that core of tumorspheres (spheroids) contains cancer stem cells,...

10 November 2012 1,219 9 View

What is your experience with in situ PCR?

Do you have any experience in in situ PCR? I think it is good approach to study intratumoral heterogeneity but I do not know nothing about it. In particular, where can I find thermal cycler for in...

09 October 2012 6,365 4 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

May members post flyers about opportunities to present at a conference? If so, where to post?

May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/

08 August 2024 4,585 1 View

Hello all, Looking for international reviewer to review Ph.D thesis in wireless sensor network.Can anybody help?

My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...

07 August 2024 4,600 2 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

Would you like to join our meta analysis research team?

I am excited to announce an opportunity for dedicated researchers to join our dynamic team for an ongoing meta-analysis study. If you are passionate about research and have a keen interest in...

01 August 2024 6,737 1 View

Is the peer-reviewed publication "MedieKultur: Journal of Media and Communication Research" (E-ISSN 1901-9726, P-ISSN : 0900-9671) a legitima?

Is the peer-reviewed publication "MedieKultur: Journal of Media and Communication Research" (ISSN Online: 1901-9726, ISSN Print: 0900-9671) a legitimate and credible scholarly journal in the field...

01 August 2024 629 3 View